LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,m,r
ImmunogenPeptide(C)RQLKQMESQPLPGER,correspondingtoaminoacidresidues201-215ofmouseadenosineA2A receptor(AccessionQ60613).3rdintracellularloop.
Accession(Uniprot)NumberQ60613
GeneID11540
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat,human,andcanis–identical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.75mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ic,ih,wb
Mayalsoworkin:ifc,ip
WesternblotWesternblotanalysisofrataorticendothelialcells(lanes1,3),ratbrain(lanes2,4)andJurkat(lanes5,6)lysates:
1,2,5.Anti-Adenosine A2A Receptor Antibody(#AAR-002),(1:200).
3,4,6.Anti-AdenosineA2A ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
ImmunohistochemistryExpressionofAdenosine A2A Receptor in mousediagonalband
ImmunohistochemicalstainingofmousediagonalbandusingAnti-Adenosine A2A ReceptorAntibody(#AAR-002).A2aR (green)appearsinthebroca ofindividualneurons(arrows)andinneuropil(asterisk). DAPIisusedasthecounterstain.
ExpressionofAdenosine A2A Receptor inratlung
ImmunohistochemicalstainingofparaffinemeddedratlungsectionsusingAnti-Adenosine A2A Receptor Antibody(#AAR-002),(1:50).A2aRisexpressedintherespiratoryepitheliumofthebronchioli(Br).Notethatsmoothmuscleandendotheliuminbloodvesselsarenegative.Hematoxilinisusedasthecounterstain.
ImmunocytochemistryRatneurospheres(Benito-Munoz,M. etal. (2016) Glia 64, 1465.).
ReferencesOkusa,M.D.(2002)Am.J.Physiol.282,F10.
Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
Nakata,H.(1989)J.Biol.Chem.264,16545.
Cunha,R.A.(2001)
NeuRochem.Int.38,107.
Fredholm,B.B.etal.(2000)Naunyn-SchmiedebergsArch.Pharmacol.362,364.
Canals,M.etal.(2005)J.Neurochem.92,337.
Ohta,A.andSitkovsky,M.(2001)Nature414,916.
Ohta,A.etal.(2006)Proc.Natl.Acad.Sci.103,13132.
ScientificbackgroundAdenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA1 Receptor(A1AR),AdenosineA2A Receptor(A2AAR),AdenosineA2B Receptor(A2BAR),andAdenosineA3 Receptor(A3AR).Allareintegralmembraneproteinsandaremembersofthe Gprotein-coupled receptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellular-NH2 terminus,cytoplasmic-COOHterminus,andathirdintracellularloopimportantforbindingGproteins.1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs.1-3
Adenosinereceptorscontrolneurotransmitterreleasethroughthe facilitatory A2AARandtheinhibitoryA1AR.4 A2AARandA1ARarethemajoradenosinereceptorsubtypesexpressedinthecentralnervoussystem(CNS).A2AARismainlyexpressedinthestriatumonGABAergicstriatopallidalneurons,whileA1ARiswidelydistributedthroughouttheCNS.5,6
A2AARwassuggestedtoplayacriticalroleinattenuationofsystemicinflammatoryresponsesandpreventionofextensivetissuedamage.7 ItwassuggestedthatextracellularadenosinethataccumulatesininflamedanddamagedtissuemayactivatetheA2AARexpressedinimmunecellsleADIngtotermination/inhibitionoftheimmuneresponse.7 ItwasfurthersuggestedthatthissamemechanismmayprotecttumorsfromantitumorTcellsthroughanimmunosuppressivesignalgeneratedbytheactivationofA2AARonTcellsbyextracellularadenosineproducedfromhypoxiccanceroustissues.8
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:17/02/2019
Anti-Adenosine A2A ReceptorAntibody(#AAR-002)isahighlyspecificantibodydirectedagainstanepitopeofmouseprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,andimmunocytochemistryapplications.IthasbeendesignedtorecognizeA2aRfromhuman,rat,andmousesamples.
Forresearchpurposesonly,notforhumanuse
WesternblotWesternblotanalysisofrataorticendothelialcells(lanes1,3),ratbrain(lanes2,4)andJurkat(lanes5,6)lysates:
1,2,5.Anti-Adenosine A2A Receptor Antibody(#AAR-002),(1:200).
3,4,6.Anti-AdenosineA2A ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
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Alomone英文简介：
Alomone Labs, your partner in ion channel research, is a leading supplier of Ion Channel, GPCR and Neural Signaling tools. We specialize in the production of primary antibodies, small molecules, proteins and peptides used in basic science research. Alomone was established in 1989 and has become the most comprehensive source of ion channel research tools used by scientists worldwide. All of Alomone`s products undergo bio***** validation and are supplied in lyophilized powder format so that you can be confident about their biological activity and long shelf life.
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