LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,m,r
ImmunogenPeptide(C)DYTWNKDNWTFSP,correspondingtoaminoacidresidues72-84ofhumanGPR65(AccessionQ8IYL9).Extracellular,1stloop.
Accession(Uniprot)NumberQ8IYL9
GeneID8477
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyMouse–12/13aminoacidresiduesidentical;rat-11/13aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
LabelFluoresceinisothiocyanate(FITC).
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution50µldoubledistilledwater(DDW).
Antibodyconcentrationafterreconstitution1mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AGR-043),anddirectflowcytometry(labeledantibody).
Applications:fc
Mayalsoworkin:ic,lci
DirectflowcytometryDirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:
___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody (#AGR-043-F),(5µg).
DirectflowcytometryofliveintactmouseM1myeloidleukemiacells:
___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody(#AGR-043-F),(5µg).
ReferencesJustus,C.R.etal.(2013)Front.Physiol.4,354.
Justus,C.R.etal.(2017)J.Transl.Med.15,204.
ScientificbackgroundGPR65,alsocalledTDAG8(T-celldeathassociatedgene8protein),isapH-sensingG-proteincoupledreceptor(GPCR)activatedbyacidicextracellularpHthroughtheprotonationofseveralhistidineresiduesinthereceptor’ssequence.GPR65isinvolvedincancercellmetastasisandproliferation,immunecellfunction,inflammation,andbloodvesselformation1.
HumanGPR65genehasbeenmappedtoalocationthatisassociatedwithTcelllymphomaandleukemiaabnormalities.
GPR65isprimarilyexpressedinimmunecellsandleukocyte-richtissuessuchascirculatingperipheralleukocytes,spleen,thymus,andtonsils.Expressionisalsodetected pain relevantlocisuchasthedorsalrootganglianeuronsandparticularlysmalldiameterneuronsresponsIBLefornociception1,2.
GPR65hastheABIlitytoaffecttumordevelopmentandgrowth.OverexpressionofGPR65inLewislungcarcinomacellsincreasestumorgrowthinmiceandmayfacilitateresistancetoacidosis-mediatedcelldeath invitro throughproteinkinaseA(PKA)andERKrelatedpathways1.
KnockdownofGPR65inNCI-H460humannon-smallcelllungcancercellsdecreasescellsurvivalinacidiccondition.AnotherstudyshowsthatactivationofGPR65byacidosiscanpromoteevasionofcellapoptosisunderglutaminestarvationanditsoverexpressionhasbeenreportedtotransformimmortalizedmammaryepithelialcells1.
LyophilizedPowderControlAntigenIncluded
Lastupdate:20/03/2019
Anti-GPR65(TDAG8)(extracellular)Antibody(#AGR-043)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot, immunohistochemistry,andlivecellflowcytometry.IthasbeendesignedtorecognizeGPR65fromrat,mouse,andhumansamples.
Anti-GPR65(TDAG8)(extracellular)-FITCAntibody(#AGR-043-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.
Forresearchpurposesonly,notforhumanuse
DirectflowcytometryDirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:
___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody (#AGR-043-F),(5µg).
DirectflowcytometryofliveintactmouseM1myeloidleukemiacells:
___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody(#AGR-043-F),(5µg).
ReferencesJustus,C.R.etal.(2013)Front.Physiol.4,354.
Justus,C.R.etal.(2017)J.Transl.Med.15,204.
Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列**的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、 神经信号通路研究（包括一些用于神经营养因子研究的*重要的抗体和生长因子）。
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Alomone Labs, your partner in ion channel research, is a leading supplier of Ion Channel, GPCR and Neural Signaling tools. We specialize in the production of primary antibodies, small molecules, proteins and peptides used in basic science research. Alomone was established in 1989 and has become the most comprehensive source of ion channel research tools used by scientists worldwide. All of Alomone`s products undergo bio***** validation and are supplied in lyophilized powder format so that you can be confident about their biological activity and long shelf life.
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