LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:m,r
Mayalsoworkin:h
ImmunogenPeptide(C)NGSRAPDHDVTQERDE,correspondingtoaminoacidresidues15-30ofmouseADRB2 (AccessionP18762).Extracellular,N-terminus.
Accession(Uniprot)NumberP18762
GeneID11555
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyHuman-15/16aminoacidresiduesidentical;rat-14/16aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.7mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ih,wb
Mayalsoworkin:ic,ifc,ip,lci
WesternblotWesternblotanalysisofratheart(lanes1and3)andlung(lanes2and4)lysates:
1,3.Anti-β2-AdrenergicReceptor(extracellular) Antibody(#AAR-016),(1:200).
2,4.Anti-β2-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
ImmunohistochemistryExpressionof β2-Adrenoceptorinratlung
Immunohistochemicalstainingofparaffin-embeddedsectionofratlungusingAnti-β2-AdrenergicReceptor(extracellular) Antibody(#AAR-016),(1:100).β2-Adrenoceptorisexpressedintherespiratoryepithelium.Hematoxilinisusedasthecounterstain.
ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Pullar,C.E.etal.(2006)FASEBJ.20,76.
Oostendorp,J.etal(2005)Am.J.Respir.Crit.CareMed.172,322.
Giembycz,M.A.andNewton,R.(2006)Eur.Respir.J.27,1286.
ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.
AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.
Adrenoceptorsaredividedintothreetypes:α1,α2 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β3.1,2 Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.1,2
β2-Adrenoceptorsaremainlypostsynapticreceptors.Theyareexpressedonanumberoftissuesincludingbloodvessels,bronchi,gastrointestinaltract(GIT),skeletalmuscle,liverandmastcell.3 Functionalβ2-adrenoceptorswerealsofoundonlymphocytes,macrophagesandneutrophils.4,5 Theyarealsotheonlyβ-adrenoceptorsubtypeexpressedinkeratinocytes,fibroblastsandmelanocytes.3 Activationofβ2-adrenoceptorsresultsinvasodilation,relaxationoftheGIT,inhibitionofhistaminereleasefrommastcellsandbronchodilation.SelectiveagoNISTsofβ2-adrenoceptorsareusedtotreatasthmaandotherrelatedbronchospasticconditionssuchasChronicobstructivepulmonarydisease(COPD).5
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:01/01/2019
Anti-β2-AdrenergicReceptor(extracellular)Antibody(#AAR-016)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthemouseβ2-adrenoceptor.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeβ2ARfrommouse,rat,andhumansamples.
Forresearchpurposesonly,notforhumanuse
WesternblotWesternblotanalysisofratheart(lanes1and3)andlung(lanes2and4)lysates:
1,3.Anti-β2-AdrenergicReceptor(extracellular) Antibody(#AAR-016),(1:200).
2,4.Anti-β2-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
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Alomone英文简介：
Alomone Labs, your partner in ion channel research, is a leading supplier of Ion Channel, GPCR and Neural Signaling tools. We specialize in the production of primary antibodies, small molecules, proteins and peptides used in basic science research. Alomone was established in 1989 and has become the most comprehensive source of ion channel research tools used by scientists worldwide. All of Alomone`s products undergo bio***** validation and are supplied in lyophilized powder format so that you can be confident about their biological activity and long shelf life.
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