LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,r
Mayalsoworkin:m
ImmunogenPeptide(C)KNANFTGPNQTSSNS,correspondingtoaminoacidresidues21-35ofhumanα1B-adrenoceptor(AccessionP35368).Extracellular,N-terminus.
Accession(Uniprot)NumberP35368
GeneID147
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyMouse-14/15aminoacidresiduesidentical;rat-13/15aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.8mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ic,ifc,ih,lci,wb
Mayalsoworkin:ip
WesternblotWesternblotanalysisofα1B-adrenoreceptorinlysatesofratbrain(lanes1and3),ratkidney(lanes2and4)andGH3cellline(lanes5and6):
1,2,5.Anti-α1B-AdrenergicReceptor(extracellular)Antibody(#AAR-018),(1:200).
3,4,6.Anti-α1B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
ImmunohistochemistryExpressionofα1B-Adrenoreceptorinratcerebellum
ImmunohistochemicalstainingoffrozensectionofratcerebellumusingAnti-α1B-AdrenergicReceptor(extracellular)Antibody (#AAR-018),(1:100).α1B-Adrenoceptor(red)isdetectedinPurkinjecells.Hoechst33342wasusedasthecounterstain.
IndirectflowcytometryIndirectflowcytometryanalysisofGH3livingcells:
___Unstainedcells.
___Cells+Anti-α1B-AdrenergicReceptor(extracellular)Antibody(#AAR-018),(10µg/5x105cells).
Thecontrolantigenisnotsuitableforthisapplication.
Livecellimaging/ImmunocytochemistryExpressionofα1B-AdrenoceptorinGH3cells
Cellsurfacedetectionof α1B-AdrenoceptorinlivingGH3cellswithAnti-α1B-AdrenergicReceptor (extracellular)Antibody (#AAR-018),followedbygoatanti-rabbit-AlexaFluor-488secondaryantibody(green).NuclearstainingofcellsusingtheDNAdyeHoechst33342(blue).
ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Perez,D.M.etal.(1993)Mol.Pharmacol.44,784.
Minneman,K.P.(1988)Pharmacol.Rev.40,87.
Schwinn,D.A.etal.(2004)MayoClin.Proc.79,1423.
ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.
Adrenergicreceptorsaremembersofthe G-proteincoupledreceptor (GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.
Adrenoceptorsaredividedintothreetypes:α1,α2 andβadrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β3.1,2 Theyareexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.1,2
Allα1-adrenoceptors(α1-ARs)activatephospholipasesCandA2.3 Inadditiontomobilizingintracellularcalcium,theα1-ARshavealsobeenshowntoactivatecalciuminfluxviavoltage-dependentand-independentcalciumchannels.4
α1B-Adrenergicreceptorpopulationsaregreatestinthespleen,kidney,cerebellum,andfetalbrain.5
α1B-Adrenoceptorcausescontractionofsmoothmusclecellsandtherebycontrolsvasculartone,bloodpressure,andacceleratesthedevelopmentofatherosclerosis.5
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea50µlfreetrialsample!
Lastupdate:19/03/2019
Anti-α1B–AdrenergicReceptor(extracellular)Antibody(#AAR-018)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanα1B-adrenoceptor.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimagingandindirectflowcytometryapplications.Ithasbeendesignedtorecognizeα1B-adrenoceptorfromhuman,ratandmousesamples.
Forresearchpurposesonly,notforhumanuse
WesternblotWesternblotanalysisofα1B-adrenoreceptorinlysatesofratbrain(lanes1and3),ratkidney(lanes2and4)andGH3cellline(lanes5and6):
1,2,5.Anti-α1B-AdrenergicReceptor(extracellular)Antibody(#AAR-018),(1:200).
3,4,6.Anti-α1B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
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Alomone英文简介：
Alomone Labs, your partner in ion channel research, is a leading supplier of Ion Channel, GPCR and Neural Signaling tools. We specialize in the production of primary antibodies, small molecules, proteins and peptides used in basic science research. Alomone was established in 1989 and has become the most comprehensive source of ion channel research tools used by scientists worldwide. All of Alomone`s products undergo bio***** validation and are supplied in lyophilized powder format so that you can be confident about their biological activity and long shelf life.
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