Saporin is obtained from the seeds of the Soapwort plant (Saponaria officinalis), a plant that grows wildly in Britain and other parts of Europe. Saporin is a plant enzyme with N-glycosidase activity that depurinates a specific nucleotide in the ribosomal RNA 28S, thus irreversibly blocking protein synthesis. It belongs to the well-characterized family of ribosome-inactivating proteins (RIPs). There are two types of RIPs: type I, which are much less cytotoxic due to the lack of the B chain and type II, which are distinguished from type I RIPs by the presence of the B chain and their ability to enter cells on their own. However, type I RIPs can still be internalized by fluid-phase endocytosis. In the case of saporin, it was reported that saporin first binds to the alpha2-macroglobulin receptor on human cells and is then internalized to the cytosol. Upon internalization, the ribosomes are inactivated, resulting in cell death.

Saporin (molecular weight 30 kDa) has no known specificity. Saporin is purified from the seeds of the Soapwort plant (Saponaria officinalis). The product is routinely tested for activity by a protein synthesis inhibition assay.

Saporin serves as a control for targeted saporin immunotoxins or ligand toxins.

To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols

Advanced Targeting Solutions（ATS）是一家位于圣地亚哥的生物技术公司，致力于为科学研究和药物开发提供高质量的靶向试剂。ATS被称为“萨波林人”，最初致力于为神经科学研究界提供研究工具。多年来，这些工具已用于科学研究和药物开发的所有领域。公司目前的产品线包括针对性毒素，抗体和定制服务，旨在帮助科学家研究系统功能，细胞功能，疾病和病症。ATS率先将靶向结合物用于“分子手术”技术。这些产品包括针对胆碱能性前脑神经元，去甲肾上腺素能和肾上腺素能神经元，巨噬细胞和小胶质细胞的特定损伤剂，以及能侵袭所有神经元上表达的抗原决定簇的泛神经元剂。此外，ATS还出售“次级结合物”，使用户可以将自己的靶向剂转化为特定的细胞毒性工具，并筛选抗体进行内在化。ATS已商业化，并提供了由Douglas Lappi博士开发和完善的Saporin技术平台的访问权限。拉皮博士与医学博士Ronald Wiley合作。在他们的学术实验室中，以验证使用主要的皂素结合物192-IgG-SAP来建立阿尔茨海默氏病模型。丹妮丝·希金斯（Denise Higgins）将她的业务专长和国际销售/营销经验带到了团队中，并将继续致力于将这一创新技术带给全球科学家。