| Protein PurificationBaculovirus ExpressionE. coli ExpressionMolecular BiologyProducts | Products: Kinases: PAK4KD PAK4KD (recombinant human PAK4 kinase domain) | Catalog #: | Size: | Concentration: | Price: | | | K0107S | 10 µg | 1 mg/ml | $375 | | | K0107M | 100 µg | 1 mg/ml | $1,375 | | | K0107L | 1 mg | 1 mg/ml | $5,640 | | | K0107B | Bulk | 1 mg/ml | Inquiry |
| To Order: | Please email PO to order@accelagen.com or fax PO to 888-239-6490 | | PRODUCT DETAIL | DATA SHEET | Sequence: GPHMSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRD YQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLT HDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKM IRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRT - The first 4 residues GPHP are from Turbo3C cleavage site.
- Phosphorylated at S474.
Component and Formulation:PAK4KD: 1 mg/ml in Storage Buffer of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT. Activity: | Specific activity:5,595 pmoles/min/µg Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen): - Different concentrations of PAK4KD were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 mM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
- Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at room temperature.
- Fluorescence was detected using lexc=460±40 nm and lem=528±20 nm filters.
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SDS-PAGE Analysis: | |
