Protein PurificationBaculovirus ExpressionMammalian ExpressionE. coli ExpressionMolecular BiologyProducts
Products: Eznyme Toolbox: TurboLigation Kit
TurboLigationTM Kit
Catalog #:
Size:
Price:
L0110S
100 reactions
$150
L0110M
500 reactions
$574
L0110B
Bulk
Inquiry
To Order:
Please email PO to order@accelagen.com or fax PO to 888-239-6490
PRODUCT DETAIL
DATA SHEET
PROTOCOL
TurboLigaseTM Unit Definition:0.015 Weiss unit (1 cohesive end ligation unit) is defined as the amount of TurboLigaseTM required to give 50% ligation of HindIII fragments of l DNA (5´ DNA termini concentration of 0.15 µM) in a total reaction volume of 20 µl in 30 minutes at 16°C in a buffer of 50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mM ATP, 10 mM dithiothreitol.TurboLigaseTM Specific Activity and Purity:TurboLigase has a specific activity >12,000 unit/mg.TurboLigase is purified to homogeneity.