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蚂蚁淘在线 / 品牌中心 / Absolute Antibody / absoluteantibody/Ab00802-23.0 Anti-Fas [R-125224]/Ab00802-23.0/200 µg
商品描述
Description:RecombinantmonoclonalantibodytoFas.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridomaR-125224.
CloneNumber:R-125224
UniProtAccessionNumberofTargetProtein:P25445
PublishedApplication(s):Activate;imaging;ELISA;FC
PublishedSpeciesReactivity:Human
Immunogen:R-125224isgeneratedbythehumanizationofthemurineHFE7Aanti-FasantibodybygraftingtheCDRregionstotheframeworkregionsofthehuman8E10antibodyandsubstitutingkeyframeworkresiduesfromthemurineantibodyintothe8E10sequence.TheoriginalHFE7AwasderivedfromahybridomacelllinegeneratedbythefusionofNS1myelomacellswithsplenocytesfromFas-deficientmicewhichhadbeenimmunizedwithpartiallypurifiedrecombinanthumanFas-AIC2AchimeraproteinconsistingoftheextracellularregionofhumanFasantigen(aa-16to150)andtheextracellularregionofthemurineIL-3receptorAIC2(aa3-423).TheHFE7AhybridomawasselectedafterscreeningbyflowcytometryfortheproductionofantibodieswiththeABIlitytobindtotheWR19L12atransformedmurineTcelllymphomacelllineexpressinghumanFasortheL5178YA1celllineexpressingmurineFas,butnottotheparentalWR19LorL5178Ycells.
Specificity:R-125224bindstotheextracellularportionofhumanFasataneptiopeconsistingofthesequenceRTQNTKCRCK(aa105-114)(pmid:11754745).FasisatypeImembraneproteinwhichbelongstothetumornecrosisfactor(TNF)receptor/nervegrowthfactor(NGF)receptorsuperfamily.ItisabletotransduceapoptoticsignalsintothecellwhenboundbyitsligandFasL(Fasligand),whichisprimarilyexpressedinactivatedTlymphoid-myeloidlineagecells,intheeye,inreproductiveorgansandinsometumors.TheFas-FasLsystemisknowntoplayanimportantroleinmaintainingtheimmunesystemasmicewithFas-defectivelymphoproliferation(lpr)andFasL-defectivegeneralizedlymphoproliferativedisease(gld)mutationsdevelopmassivelymphadenopathyandautoimmunediseases.
ApplicationNotes:R-125224showsthesamebindingaffinityandthesameabilitytoinduceapoptosisinWR19L12acellsthatexpresshumanFasastheparentalmurineHFE7Aantibody.R-125224selectivelyinducesapoptosisintypeIactivatedlymphocytesbutnotintypeIIcells.R-125224isabletoinduceapoptosisinthehumanlymphoidcelllinesH9andSKW6.4,aswellasactivatedhumanlymphocytes,whencross-linkedwithanti-hIgGsecondaryantibodies.TheantibodyisunabletoinduceapoptosisinHPB-ALLcells,Jurkatcellsorhumanhepatocytes.R-125224hasbeenusedinvivowhereithasbeenshowntogreatlyreducethenumberofactivatedhumanhumanCD3+Fas+TcellsinaSCIDmousemodelpossessingafunctionalhumanimmunesystem.Fasantigentissuedistributionincynomolgusmonkeyswithcollagen-inducedarthritisatthearmjoint(CIAmonkeys)hasbeenstudiedusing[125I]-LabeledR-125224.HighrADIoactivityinthebonemarrow,thymus,lungs,liver,adrenals,spleen,ovaries,axillarylymphnodeandmesentericlymphnodecomparedtotheradioactivityintheplasmawasobserved,whichcorrelateswithFasexpression.FascanalsobedetectedbyR-125224byELISA.
AntibodyFirstPublishedin:
HaruyamaetalHumanizationoftheMouseAnti-FasAntibodyHFE7AandCrystalStructureoftheHumanizedHFE7AFabFragmentBiol.Pharm.Bull.25(12)1537—1545(2002)PMID:12499636Noteonpublication:Describesthegenerationof5humanizedversionsofthemouseHFE7Aantibodyandtheirpropertieswerestudiedandcomparedwiththeparentalantibody.ThecrystalstructureofR-125224wasdetermined.
Concentration:Seeviallabel.
Importantnote–Thisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals. Absolute Antibody我们现在与Digital Proteomics合作提供多克隆永生化服务。这意味着您可以生产可复制且不受限制的多克隆试剂，或者利用天然免疫系统来识别新的抗体候选物。对于这项服务，您可以提供免疫宿主生物的血清和B细胞，纯化的抗原用于免疫，或者只是告诉我们您的靶标，我们将做剩下的事情。鉴于其中之一，我们可以提供多达10条由发现引擎选择的重链序列和10条轻链序列，在交互式报告中提供具有蛋白质组学证据的扩展库，根据要求将抗体工程化为任何种类和同种型，以及我们提供的抗体表征数据获得。此服务是100％免版税的。 ThischimericrabbitantibodywasmadeusingthevariabledomainsequencesoftheoriginalHumanIgG1format,forimprovedcompatibilitywithexistingreagents,assaysandtechniques.
SpeciesandIsotype:RabbitIgG,kappaCloneNumber:R-125224
UniProtAccessionNumberofTargetProtein:P25445
PublishedApplication(s):Activate;imaging;ELISA;FC
PublishedSpeciesReactivity:Human
Immunogen:R-125224isgeneratedbythehumanizationofthemurineHFE7Aanti-FasantibodybygraftingtheCDRregionstotheframeworkregionsofthehuman8E10antibodyandsubstitutingkeyframeworkresiduesfromthemurineantibodyintothe8E10sequence.TheoriginalHFE7AwasderivedfromahybridomacelllinegeneratedbythefusionofNS1myelomacellswithsplenocytesfromFas-deficientmicewhichhadbeenimmunizedwithpartiallypurifiedrecombinanthumanFas-AIC2AchimeraproteinconsistingoftheextracellularregionofhumanFasantigen(aa-16to150)andtheextracellularregionofthemurineIL-3receptorAIC2(aa3-423).TheHFE7AhybridomawasselectedafterscreeningbyflowcytometryfortheproductionofantibodieswiththeABIlitytobindtotheWR19L12atransformedmurineTcelllymphomacelllineexpressinghumanFasortheL5178YA1celllineexpressingmurineFas,butnottotheparentalWR19LorL5178Ycells.
Specificity:R-125224bindstotheextracellularportionofhumanFasataneptiopeconsistingofthesequenceRTQNTKCRCK(aa105-114)(pmid:11754745).FasisatypeImembraneproteinwhichbelongstothetumornecrosisfactor(TNF)receptor/nervegrowthfactor(NGF)receptorsuperfamily.ItisabletotransduceapoptoticsignalsintothecellwhenboundbyitsligandFasL(Fasligand),whichisprimarilyexpressedinactivatedTlymphoid-myeloidlineagecells,intheeye,inreproductiveorgansandinsometumors.TheFas-FasLsystemisknowntoplayanimportantroleinmaintainingtheimmunesystemasmicewithFas-defectivelymphoproliferation(lpr)andFasL-defectivegeneralizedlymphoproliferativedisease(gld)mutationsdevelopmassivelymphadenopathyandautoimmunediseases.
ApplicationNotes:R-125224showsthesamebindingaffinityandthesameabilitytoinduceapoptosisinWR19L12acellsthatexpresshumanFasastheparentalmurineHFE7Aantibody.R-125224selectivelyinducesapoptosisintypeIactivatedlymphocytesbutnotintypeIIcells.R-125224isabletoinduceapoptosisinthehumanlymphoidcelllinesH9andSKW6.4,aswellasactivatedhumanlymphocytes,whencross-linkedwithanti-hIgGsecondaryantibodies.TheantibodyisunabletoinduceapoptosisinHPB-ALLcells,Jurkatcellsorhumanhepatocytes.R-125224hasbeenusedinvivowhereithasbeenshowntogreatlyreducethenumberofactivatedhumanhumanCD3+Fas+TcellsinaSCIDmousemodelpossessingafunctionalhumanimmunesystem.Fasantigentissuedistributionincynomolgusmonkeyswithcollagen-inducedarthritisatthearmjoint(CIAmonkeys)hasbeenstudiedusing[125I]-LabeledR-125224.HighrADIoactivityinthebonemarrow,thymus,lungs,liver,adrenals,spleen,ovaries,axillarylymphnodeandmesentericlymphnodecomparedtotheradioactivityintheplasmawasobserved,whichcorrelateswithFasexpression.FascanalsobedetectedbyR-125224byELISA.
AntibodyFirstPublishedin:
HaruyamaetalHumanizationoftheMouseAnti-FasAntibodyHFE7AandCrystalStructureoftheHumanizedHFE7AFabFragmentBiol.Pharm.Bull.25(12)1537—1545(2002)PMID:12499636Noteonpublication:Describesthegenerationof5humanizedversionsofthemouseHFE7Aantibodyandtheirpropertieswerestudiedandcomparedwiththeparentalantibody.ThecrystalstructureofR-125224wasdetermined.
ProductForm
Purification:ProteinAaffinitypurifiedSuppliedin:PBSwith0.02%Proclin300.
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°CConcentration:Seeviallabel.
