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蚂蚁淘在线 / 品牌中心 / Absolute Antibody / absoluteantibody/Ab00230-10.0 Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]/Ab00230-10.0/ 200 µg
商品描述
Description:RecombinantmonoclonalantibodytoFlavivirusgroupantigen.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridomaD1-4G2-4-15(4G2).
CloneNumber:D1-4G2-4-15(4G2)
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):IF;WB;NTRL;ELISA;FC;IHC-P
PublishedSpeciesReactivity:DengueVirus;ZikaVirus;WestNileVirus;YellowFeverVirus;Flaviviridae
Immunogen:DengueVirustype2antigens.
Specificity:Recognisesflavivirusgroupspecificantigens(Denguevirus,WestNileVirus,JapaneseEncephalitis,YellowFeverVirus,Zikavirusetc).ItbindstothefusionloopattheextremityofdomainIIofproteinE.
ApplicationNotes:Thisantibodybindstoflavivirusgroupantigen,proteinE.Itcanbeusedasananti-Denguevirusantibody,anti-WestNilevirusantibody,anti-JapaneseEncephalitis,anti-YellowFeverVirusoranti-ZikaVirusantibody(Aubryetal.2016)toidentifycellsinfectedwiththeseflaviviridae.ItbindstothefusionloopattheextremityofdomainIIofEproteinfromallfourSEROtypesandpreventssyncytiaformation(Summers,1989).TheepitopeishighlyconservedamongstflaviviridaeandhasbeenfunctionallyanalyzedindetailbyCrillandChang2004(PMID:15564505).Previousstudieshaveusedacetone-(Henchaletal.1982)ormethanol-fixedslides(Moreland&Tay,2010).PleasenotethatbindingofthisantibodyhasbeenreportedtobesensitivetoreductionandWesternBlotsshouldbeperformedundernon-reducingconditions(Laietal.2008).Aversionof4G2directlyconjugaedtoAPCwasusedbyQuickeandco-workers(Quicke,2016)todetectZikavirusinhumanplacentalmacrophagesbyflow-cytometry(Quicke2016).Ramaiahetal.(2016)usedAb00230-2.0ata1:200dilutiontostainforZikavirusinmethanol-fixedVerocells48hpost-infection.
AntibodyFirstPublishedin:
Nawaetal.DevelopmentofdengueIgM-captureenzyme-linkedimmunosorbentassaywithhighersensitivityusingmonoclonaldetectionantibody.JVirolMethodsPMID:11164919
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
Importantnote–Thisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals. Absolute Antibody我们现在与Digital Proteomics合作提供多克隆永生化服务。这意味着您可以生产可复制且不受限制的多克隆试剂，或者利用天然免疫系统来识别新的抗体候选物。对于这项服务，您可以提供免疫宿主生物的血清和B细胞，纯化的抗原用于免疫，或者只是告诉我们您的靶标，我们将做剩下的事情。鉴于其中之一，我们可以提供多达10条由发现引擎选择的重链序列和10条轻链序列，在交互式报告中提供具有蛋白质组学证据的扩展库，根据要求将抗体工程化为任何种类和同种型，以及我们提供的抗体表征数据获得。此服务是100％免版税的。 ThischimerichumanantibodywasmadeusingthevariabledomainsequencesoftheoriginalmouseIgG2aformat,forimprovedcompatibilitywithexistingreagents,assaysandtechniques.
SpeciesandIsotype:HumanIgG1,kappaCloneNumber:D1-4G2-4-15(4G2)
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):IF;WB;NTRL;ELISA;FC;IHC-P
PublishedSpeciesReactivity:DengueVirus;ZikaVirus;WestNileVirus;YellowFeverVirus;Flaviviridae
Immunogen:DengueVirustype2antigens.
Specificity:Recognisesflavivirusgroupspecificantigens(Denguevirus,WestNileVirus,JapaneseEncephalitis,YellowFeverVirus,Zikavirusetc).ItbindstothefusionloopattheextremityofdomainIIofproteinE.
ApplicationNotes:Thisantibodybindstoflavivirusgroupantigen,proteinE.Itcanbeusedasananti-Denguevirusantibody,anti-WestNilevirusantibody,anti-JapaneseEncephalitis,anti-YellowFeverVirusoranti-ZikaVirusantibody(Aubryetal.2016)toidentifycellsinfectedwiththeseflaviviridae.ItbindstothefusionloopattheextremityofdomainIIofEproteinfromallfourSEROtypesandpreventssyncytiaformation(Summers,1989).TheepitopeishighlyconservedamongstflaviviridaeandhasbeenfunctionallyanalyzedindetailbyCrillandChang2004(PMID:15564505).Previousstudieshaveusedacetone-(Henchaletal.1982)ormethanol-fixedslides(Moreland&Tay,2010).PleasenotethatbindingofthisantibodyhasbeenreportedtobesensitivetoreductionandWesternBlotsshouldbeperformedundernon-reducingconditions(Laietal.2008).Aversionof4G2directlyconjugaedtoAPCwasusedbyQuickeandco-workers(Quicke,2016)todetectZikavirusinhumanplacentalmacrophagesbyflow-cytometry(Quicke2016).Ramaiahetal.(2016)usedAb00230-2.0ata1:200dilutiontostainforZikavirusinmethanol-fixedVerocells48hpost-infection.
AntibodyFirstPublishedin:
Nawaetal.DevelopmentofdengueIgM-captureenzyme-linkedimmunosorbentassaywithhighersensitivityusingmonoclonaldetectionantibody.JVirolMethodsPMID:11164919
ProductForm
Purification:ProteinAaffinitypurifiedSuppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
