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蚂蚁淘在线 / 品牌中心 / Absolute Antibody / absoluteantibody/Ab00337-24.1 Anti-Rhodopsin [Rho 1D4]/Ab00337-24.1/ 200 µg
商品描述
Description:RecombinantmonoclonalantibodytoRhodopsin.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridomaRho1D4.
CloneNumber:Rho1D4
UniProtAccessionNumberofTargetProtein:P08100
AlternativeName(s)ofTarget:VisualPurple
PublishedApplication(s):RADIoimmunelabeling;WB;ELISA;IP;IF;IHC
PublishedSpeciesReactivity:Mouse;Rat;Cow;Human,Zebrafish,Amphibians
Immunogen:Bleachedbovinerodoutersegment(ROS).
Specificity:TheantibodybindstotheC-terminusofrhodopsinonthecytoplasmicsideofdiskmembranes.Theepitopehasthefollowingaminoacidsequence:"TETSQVAPA".Alimitationofthe1D4tag,however,isthatithastobeplacedattheC-terminusofaprotein.ThisisbecausetheRho1D4monoclonalantibodyrequiresafreecarboxylategroupforhighaffinitybinding.AmidationofthecarboxylgrouplowersitsimmunoreactivitytotheRho1D4antibodybyover100fold.
ApplicationNotes:TheantibodybindstotheC-terminusofrhodopsinonthecytoplasmicsideofdiskmembranes.RhodopsinisthemajorpigmentinrodphotoreceptorsandbelongstothefamilyofG-protein-coupledreceptors(GPCRs).Thelinearepitope(TETSQVAPA)canbeusedasaproteintag.
AntibodyFirstPublishedin:
Moldayetal.1983Monoclonalantibodiestorhodopsin:characterization,corss-reactivity,andapplicatoinassturcturalprobes.Biochemistry,1983;22(3):653-660PMID:6188482Noteonpublication:Describesthegenerationoftwomonoclonalantibodiesfromhybridoma.
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
Importantnote–Thisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals. Absolute Antibody我们现在与Digital Proteomics合作提供多克隆永生化服务。这意味着您可以生产可复制且不受限制的多克隆试剂，或者利用天然免疫系统来识别新的抗体候选物。对于这项服务，您可以提供免疫宿主生物的血清和B细胞，纯化的抗原用于免疫，或者只是告诉我们您的靶标，我们将做剩下的事情。鉴于其中之一，我们可以提供多达10条由发现引擎选择的重链序列和10条轻链序列，在交互式报告中提供具有蛋白质组学证据的扩展库，根据要求将抗体工程化为任何种类和同种型，以及我们提供的抗体表征数据获得。此服务是100％免版税的。 ThischimericgoatantibodywasmadeusingthevariabledomainsequencesoftheoriginalmouseIgG1formatforimprovedcompatibilitywithexistingreagents,assaysandtechniques.
SpeciesandIsotype:GoatIgG,kappaCloneNumber:Rho1D4
UniProtAccessionNumberofTargetProtein:P08100
AlternativeName(s)ofTarget:VisualPurple
PublishedApplication(s):RADIoimmunelabeling;WB;ELISA;IP;IF;IHC
PublishedSpeciesReactivity:Mouse;Rat;Cow;Human,Zebrafish,Amphibians
Immunogen:Bleachedbovinerodoutersegment(ROS).
Specificity:TheantibodybindstotheC-terminusofrhodopsinonthecytoplasmicsideofdiskmembranes.Theepitopehasthefollowingaminoacidsequence:"TETSQVAPA".Alimitationofthe1D4tag,however,isthatithastobeplacedattheC-terminusofaprotein.ThisisbecausetheRho1D4monoclonalantibodyrequiresafreecarboxylategroupforhighaffinitybinding.AmidationofthecarboxylgrouplowersitsimmunoreactivitytotheRho1D4antibodybyover100fold.
ApplicationNotes:TheantibodybindstotheC-terminusofrhodopsinonthecytoplasmicsideofdiskmembranes.RhodopsinisthemajorpigmentinrodphotoreceptorsandbelongstothefamilyofG-protein-coupledreceptors(GPCRs).Thelinearepitope(TETSQVAPA)canbeusedasaproteintag.
AntibodyFirstPublishedin:
Moldayetal.1983Monoclonalantibodiestorhodopsin:characterization,corss-reactivity,andapplicatoinassturcturalprobes.Biochemistry,1983;22(3):653-660PMID:6188482Noteonpublication:Describesthegenerationoftwomonoclonalantibodiesfromhybridoma.
ProductForm
Purification:ProteinAaffinitypurifiedSuppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
