- 关注来自人类患者的重组抗体
- Anti-Virus Antibodies
- 研究级生物仿制药
- Recombinant Isotype Controls
- 表位标记抗体
- Antibodies to Epitope Tags
- 抗过敏原抗体
- Primary Antibodies
- 体内研究用重组抗体
- 重组F4/80克隆CI:A3-1
- Recombinant F4/80 Clone CI:A3-1
- 重组免疫球蛋白Fc结构域（Ig-Fc结构域）
- 其他产品
- Recombinant Antibodies from Human Patients
- 同型对照
- 人源性重组抗体
- 多标签控制蛋白
- Fc融合蛋白
- 抗病毒抗体
- 重组自由轻链
- Research-Grade Biosimilars
UniProtAccessionNumberofTargetProtein:Q8R183
AlternativeName(s)ofTarget:TCellImmunoglobulinMucin-2;TCellimmunoglobulinandmucindomainprotein2;TIM3Immunogen:ThisantibodywasraisedbyimmunisingSpragueDawleyratswithTIM-2-Igconsistingoftheextracellulardomain(aa1-230ofmouseTIM-2)andtheFcportionofmouseIgG2a,emulsifiedincompleteFreund"sadjuvantandthenfusinglymphnodecellsfromtheimmunizedratswithP3U1myelomacells.
Specificity:ThisantibodyisspecificformurineTIM-2,atransmembraneproteinthatisexpressedonactivatedTcellsandpreferentiallyondifferentiatedTh2cells.AblockadeofTIM-2/TIM-2ligandinteractionresultsinTcellhyperproliferationandTh2cytokineproduction.TIM-2playsacriticalroleinthedevelopmentofatopicdiseaseandotherTh2-biasedimmuneresponses.
ApplicationNotes:ThisantibodyhasbeenusedinvariousFACSanalyses,forinstance,toevaluatetheexpressionofTIMfamilymembersonmousemastcells(Nakaeetal,2007)andtoblocktheH-ferritinbindingtoTIM-2/L5178Ycells(Kawamotoetal,2011).ThisantibodyhasbeenusedinFACSandimmunohistochemistryanalysestoshowthatTIM-2isexpressedonmousefetalhepatocytes(Watanabeetal,2007).ThisantibodyhasalsobeenusedinWesternBlotsandforblockingexperimentsinferritinbindinganalysis,whichsuggeststhatTim-2istheH-ferritinreceptoronoligodendrocytes(Todorichetal,2008).Inaddition,thisantibodyhasbeenusedincollagen-inducedarthritis(CIA)modelstodeterminewhetherTIM-2contributestothedevelopmentofThelper(Th)1orTh17cellsandjointinflammation(Kawamotoetal,2011).
Antibodyfirstpublishedin:Nakaeetal.TIM-1andTIM-3enhancementofTh2cytokineproductionbymastcells.Blood.2007Oct1;110(7):2565–2568.PMID:17620455Noteonpublication:DescribetheoriginalgenerationofthiscloneRMT2-1,togetherwithanotherclone(RMT2-14),ofthisanti-mouseTIM-2antibodyandtheirsubsequentuseforevaluationoftheexpressionofTIMfamilymembersonmousemastcells.
