UniProt Accession Number of Target Protein: P67875

Alternative Name(s) of Target: Asp f1; Aspf1; ribotoxin Asp f 1; ribonuclease mitogillin; allergen Asp f I; Asp f I; IgE-binding ribotoxin; major allergen Asp f 1; allergen: Asp f 1Immunogen: 14H4 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion. Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 1 IgE.

Specificity: This antibody recognises Asp f 1.

Application Notes: This antibody's target protein (Asp f 1) was confirmed through IP and mass spectrometry analysis (Wurth et al., 2018). WB and ELISA: the antibody was successfull in detecting Asp f 1 antigen in a commercial Aspergillus extract ALKB (Wurth et al., 2018).

Antibody first published in:Wurth et al.Human IgE mAbs define variability in commercial Aspergillus extract allergen compositionJCI Insight. 2018 Oct 18;3(20). pii: 123387. doi: 10.1172/jci.insight.123387.PMID:30333320Note on publication:The article describes a method of generating Aspergillus-reactive IgE–secreting human hybridomas (among them these secreting this antibody). Subsequently, the antibody is characterized and its specificity is determined. Finally, this antibody is used to determin a potential presence of its antigen in the commercialy-available Aspergillus samples used in diagnosis and treatment of Aspergillus-related allergies.