Overview

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Ex/Em(nm)	544/575
MW	1128.13
CAS#	N/A
Solvent	DMSO
Storage	F/D/L
Category	EnzymeDetection Phosphodiesterases
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FluorescentlylabeledATPtracersareusefulformonitoringATP-bindingproteinsandotherbiomolecules.TheATP-TAMRAtracerwasdemonstratedtobindATPasesthatareaclassofenzymesthatcatalyzethedecompositionofATPintoADPandafreephosphateion.

Spectrum

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References&Citations

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1. LinCY,HuangJY,LoLW.(2013)Decipheringthecatalysis-associatedconformationalchangesofhumanadenylatekinase1withsingle-moleculespectroscopy.JPhysChemB,117,13947.
2. SzymanskiMR,JezewskaMJ,BujalowskiW.(2010)TheEscherichiacoliPriAhelicase-double-strandedDNAcomplex:locationofthestrongDNA-bindingsubsiteonthehelicasedomainoftheproteinandtheaffinitycontrolbythetwonucleotide-bindingsitesoftheenzyme.JMolBiol,402,344.
3. HunkeC,TadwalVS,ManimekalaiMS,RoessleM,GruberG.(2010)TheeffectofNBD-Clinnucleotide-bindingofthemajorsubunitalphaandBofthemotorproteinsF1FOATPsynthaseandA1AOATPsynthase.JBioenergBiomembr,42,1.
4. RamalingamJK,HunkeC,GaoX,GruberG,PreiserPR.(2008)ATP/ADPbindingtoanovelnucleotidebindingdomainofthereticulocyte-bindingproteinPy235ofPlasmodiumyoelii.JBiolChem,283,36386.
5. SenB,ChakrabortyA,DattaR,BhattacharyyaD,DattaAK.(2006)ReversalofADP-mediatedaggregationofadenosinekinasebycyclophilinleadstoitsreactivation.Biochemistry,45,263.
6. CochranJC,GilbertSP.(2005)ATPasemechanismofEg5intheabsenceofmicrotubules:insightintomicrotubuleactivationandallostericinhibitionbymonastrol.Biochemistry,44,16633.
7. GuoQ,ChenB,WangX.(2004)EvidenceforproximalcysteineandlysineresiduesatorneartheactivesiteofargininekinaseofStichopusjaponicus.Biochemistry(Mosc),69,1336.
8. VieyraA,MintzE,LoweJ,GuillainF.(2004)Ca2+bindingtosarcoplasmicreticulumATPasephosphorylatedbyPirevealsfourthapsigargin-sensitiveCa2+sitesinthepresenceofADP.BiochimBiophysActa,1667,103.
9. WangQ,SongC,YangX,LiCC.(2003)D1ringisstableandnucleotide-independent,whereasD2ringundergoesmajorconformationalchangesduringtheATPasecycleofp97-VCP.JBiolChem,278,32784.
10. FlowersS,BiswasEE,BiswasSB.(2003)ConformationaldynamicsofDnaBhelicaseuponDNAandnucleotidebinding:analysisbyintrinsictryptophanfluorescencequenching.Biochemistry,42,1910.

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