| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | None/None |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | EnzymeDetection HorserADIshPeroxidase(HRP) |
| Related | BiochemicalAssays |
Note1:Usebuffers,suchasHepes,Tris,Tricine,Mops,PIPESorMES,thatcontain<10%phosphatewiththisHRPConjugateStabilizer.Bufferscontaining>10%phosphateorsignificantamountsofborateandcarbonatemaycauseprecipitation.
- DilutetheprimaryorsecondaryperoxidaseconjugatedantibodyorotherproteinorcompoundwiththeHRPConjugateStabilizertothedesired1Xworkingconcentrationdeterminedforyourconjugate.
- ThisHRPconjugatestabilizerdilutesandstabilizesHRPconjugatesinasinglestep.
- DonotfreezetheHRPconjugatesoncetheHRPconjugatestabilizerisadded.ManyHRP-conjugatespreparedinthismannermaintainHRPactivityforupto12monthsorlongerwhenproperlystored.
| References&Citations |  PrinterFriendlyVersion |
1. MiuraT,MuraokaS,FujimotoY.(2003)Inactivationofcreatinekinaseinducedbyquercetinwithhorseradishperoxidaseandhydrogenperoxide.pro-oxidativeandanti-oxidativeactionsofquercetin.FoodChemToxicol,41,759.
2. SchmidtA,SchumacherJT,ReicheltJ,HechtHJ,BilitewskiU.(2002)MechaNISTicandmolecularinvestigationsonstabilizationofhorseradishperoxidaseC.AnalChem,74,3037.
3. GuY,ChenY,YeL.(1992)Electronmicroscopicaldemonstrationofhorseradishperoxidasebyuseoftetramethylbenzidineaschromogenandsodiumtungstateasstabilizer(TMB-STmethod):atracingmethodwithhighsensitivityandwellpreservedultrastructuraltissue.JNeurosciMethods,42,1.
4. CooperAM.(1990)Thehistochemicalreactionofhorseradishperoxidaseinrodent"whole-brain"preparations.ExpBrainRes,82,456.
5. JoostenEA.(1990)Anultrastructuraldouble-labellingmethod:immunohistochemicallocalizationofcelladhesionmoleculeL1onHRP-labelleddevelopingcorticospinaltractaxonsintherat.Histochemistry,94,645.
6. DederenPJ,JoostenEA.(1989)Atriple-labelingmethod:HRPanterogradetracttracingcombinedwithdoubleimmunofluorescentcellstainingindevelopingneuraltissueoftherat.JNeurosciMethods,30,71.
7. LiangFY,WanXC.(1989)ImprovementofthetetramethylbenzidinereactionwithammoniummolyBDateasastabilizerforlightandelectronmicroscopicligand-HRPneurohistochemistry,immunocytochemistryanddouble-labelling.JNeurosciMethods,28,155.
8. LiangFY,WanXC.(1989)[AmmoniummolybdateasstabilizingagentintetramethylbenzidinereactionofCB-HRPneurohistochemistryanditsapplicationtoelectronmicroscopyandimmunocytochemistry].ZhongguoYiXueKeXueYuanXueBao,11,142.
9. OluchaF,Martinez-GarciaF,Lopez-GarciaC.(1985)Anewstabilizingagentforthetetramethylbenzidine(TMB)reactionproductinthehistochemicaldetectionofhorseradishperoxidase(HRP).JNeurosciMethods,13,131.
10. AlbersFJ,KloosterJ,vanderWantJJ.(1984)Potassiumferricyanide:astabilizerforhorseradishperoxidase.JHistochemCytochem,32,1005.
