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Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | None/None |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | F/D/L |
Category | MicroBIOLOGy FluorescenceImaging |
Related | BiochemicalAssays |
1.Performformaldehydefixation:Incubatethecellswith4%formaldehydefor10-30minutesatroomtemperature.
Note:Avoidanymethanolcontainingfixativessincemethanolcandamageactinduringthefixationprocess.Thepreferredfixativeismethanol-freeformaldehyde.
2.Rinsethefixedcells2-3timesinPBS.
3.Thecellsarereadyforimmunohistochemistry/immunofluorescence.
Note:StorethefixedcellsinPBSat4oC.
References&Citations | ![]() CitationExplorer |
RhinacanthinCAlleviatesAmyloid-βFibrils’ToxicityonNeuronsandAttenuatesNeuroinflammationTriggeredbyLPS,Amyloid-β,andInterferon-γinGlialCells
Authors:Kai-AnChuang,Ming-HanLi,Ni-HsuanLin,Chih-HsuanChang,ILu,IPan,TomoyaTakahashi,Ming-DerPerng,Shu-FangWen
Journal:OxidativeMedicineandCellularLongevity(2017)
ImpactofsiRNAtargetingofβ-cateninondifferentiationofratneuralstemcellsandgeneexpressionofNgn1andBMP4followinginvitrohypoxic-ischemicbraindamage
Authors:XiaoyingZhang,CuicuiZhu,QiongLuo,JvDong,LvLiu,MinLi,HongtaoZhu,XiangpingMa,JunWang
Journal:MolecularMedicineReports(2016):3595--3601
Applicationofinterleukin-22mediatesprotectioninexperimentalacetaminophen-inducedacuteliverinjury
Authors:PatrickScheiermann,MalteBachmann,ItamarGoren,BernhardZwissler,JosefPfeilschifter,HeikoMühl
Journal:TheAmericanjournalofpathology(2013):1107--1113
Synergisticactivityofαvβ3integrinsandtheelastinbindingproteinenhancecell-matrixinteractionsonbioactivehydrogelsurfaces
Authors:DhavalPatel,SusanEVandromme,MichaelEReid,LakeshiaJTaite
Journal:Biomacromolecules(2012):1420--1428
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