Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 358/461 |
MW | 350.25 |
CAS# | 28718-90-3 |
Solvent | Water |
Storage | F/D/L |
Category | NucleicAcidDetection DNADetection |
Related | LabelingCells FluorescenceImaging BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
Usethefixationprotocolappropriateforyoursample.DAPIstainingisnormallyperformedafterallotherstaining.
Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyebindingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.AddDAPIstainusingtheconcentrationsbetween0.5and5µMandincubateitfor15to60minutesasaguide.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
References&Citations | CitationExplorer |
AstrocyticexpressionoftheCXCL12receptor,CXCR7/ACKR3isahallmarkofthediseased,butnotdevelopingCNS
Authors:MaltePuchert,FABIanPelkner,GregorStein,DoychinNAngelov,JohannesBoltze,Daniel-ChristophWagner,FrancescaOdoardi,AlexanderFlügel,WolfgangJStreit,JürgenEngele
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Cube-shapedtheranosticpaclitaxelprodrugnanocrystalswithsurfacefunctionalizationofSPCandMPEG-DSPEforimagingandchemotherapy
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Legionellapneumophilainfectionactivatesbystandercellsdifferentiallybybacterialandhostcellvesicles
Authors:AnnaLenaJung,ChristinaElenaHerkt,ChristineSchulz,KathrinBolte,KerstinSeidel,NicolettaScheller,AlexandraSittka-Stark,WilhelmBertrams,BerndSchmeck
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Peptidesmimickingviralproteinsofporcinecircovirustype2wereprofiledbythespectrumofmouseanti-PCV2antibodies
Authors:Ling-ChuHung,Cheng-YaoYang,Ivan-ChenCheng
Journal:BMCimmunology(2017):25
Poly(ADP-ribose)polymeraseinhibitorsactivatethep53signalingpathwayinneuralstem/Progenitorcells
Authors:AkikoOkuda,SuguruKurokawa,MasanoriTakehashi,AikaMaeda,KatsuyaFukuda,YukariKubo,HyumaNogusa,TomokaTakatani-Nakase,ShujiroOkuda,KunihiroUeda
Journal:BMCneuroscience(2017):14
ApoptoticeffectoftheselectivePPARβ/δagoNISTGW501516ininvasivebladdercancercells
Authors:AdelinePéchery,SylvieFauconnet,HuguesBittard,IsabelleLascombe
Journal:TumorBIOLOGy(2016):1--14
Carbonnanotube--Protaminehybrid:EvaluationofDNAcellpenetration
Authors:CécileCaoduro,RaoudhaKacem,KhaoulaBoukari,FabienPicaud,Claire-HélèneBrachais,DavidMonchaud,ChristopheBorg,HatemBoulahdour,TijaniGharbi,RégisDelage-Mourroux
Journal:Carbon(2016):742--752
IdentificationofepigeneticfactorsregulatingthemesenchymetoepitheliumtransitionbyRNAinterferencescreeninginbreastcancercells
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Legionellapneumophila-DerivedOuterMembraneVesiclesPromoteBacterialReplicationinMacrophages
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Redox-SensitiveCitronellol--CabazitaxelConjugate:MaintainedinVitroCytotoxicityandSelf-AssembledasMultifunctionalNanomedicine
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Journal:Bioconjugatechemistry(2016):1360--1372