| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 358/461 |
| MW | 350.25 |
| CAS# | 28718-90-3 |
| Solvent | Water |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
Usethefixationprotocolappropriateforyoursample.DAPIstainingisnormallyperformedafterallotherstaining.
Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyebindingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.AddDAPIstainusingtheconcentrationsbetween0.5and5µMandincubateitfor15to60minutesasaguide.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
| References&Citations |  CitationExplorer |
AstrocyticexpressionoftheCXCL12receptor,CXCR7/ACKR3isahallmarkofthediseased,butnotdevelopingCNS
Authors:MaltePuchert,FABIanPelkner,GregorStein,DoychinNAngelov,JohannesBoltze,Daniel-ChristophWagner,FrancescaOdoardi,AlexanderFlügel,WolfgangJStreit,JürgenEngele
Journal:MolecularandCellularNeuroscience(2017)
Cube-shapedtheranosticpaclitaxelprodrugnanocrystalswithsurfacefunctionalizationofSPCandMPEG-DSPEforimagingandchemotherapy
Authors:FuqiangGuo,JiajiaShang,HaiZhao,KangrongLai,YangLi,ZhongxiongFan,ZhenqingHou,GuanghaoSu
Journal:ColloidsandSurfacesB:Biointerfaces(2017)
Legionellapneumophilainfectionactivatesbystandercellsdifferentiallybybacterialandhostcellvesicles
Authors:AnnaLenaJung,ChristinaElenaHerkt,ChristineSchulz,KathrinBolte,KerstinSeidel,NicolettaScheller,AlexandraSittka-Stark,WilhelmBertrams,BerndSchmeck
Journal:ScientificReports(2017)
Peptidesmimickingviralproteinsofporcinecircovirustype2wereprofiledbythespectrumofmouseanti-PCV2antibodies
Authors:Ling-ChuHung,Cheng-YaoYang,Ivan-ChenCheng
Journal:BMCimmunology(2017):25
Poly(ADP-ribose)polymeraseinhibitorsactivatethep53signalingpathwayinneuralstem/Progenitorcells
Authors:AkikoOkuda,SuguruKurokawa,MasanoriTakehashi,AikaMaeda,KatsuyaFukuda,YukariKubo,HyumaNogusa,TomokaTakatani-Nakase,ShujiroOkuda,KunihiroUeda
Journal:BMCneuroscience(2017):14
ApoptoticeffectoftheselectivePPARβ/δagoNISTGW501516ininvasivebladdercancercells
Authors:AdelinePéchery,SylvieFauconnet,HuguesBittard,IsabelleLascombe
Journal:TumorBIOLOGy(2016):1--14
Carbonnanotube--Protaminehybrid:EvaluationofDNAcellpenetration
Authors:CécileCaoduro,RaoudhaKacem,KhaoulaBoukari,FabienPicaud,Claire-HélèneBrachais,DavidMonchaud,ChristopheBorg,HatemBoulahdour,TijaniGharbi,RégisDelage-Mourroux
Journal:Carbon(2016):742--752
IdentificationofepigeneticfactorsregulatingthemesenchymetoepitheliumtransitionbyRNAinterferencescreeninginbreastcancercells
Authors:Jean-MarcGregoire,LaurenceFleury,ClaraSalazar-Cardozo,FrédéricAlby,VéroniqueMasson,PaolaBarbaraArimondo,FrédéricAusseil
Journal:BMCcancer(2016):700
Legionellapneumophila-DerivedOuterMembraneVesiclesPromoteBacterialReplicationinMacrophages
Authors:AnnaLenaJung,CorneliaStoiber,ChristinaEHerkt,ChristineSchulz,WilhelmBertrams,BerndSchmeck
Journal:PLoSPathog(2016):e1005592
Redox-SensitiveCitronellol--CabazitaxelConjugate:MaintainedinVitroCytotoxicityandSelf-AssembledasMultifunctionalNanomedicine
Authors:PengXue,DanLiu,JingWang,NaZhang,JiahuaZhou,LinLi,WeilingGuo,MengchiSun,XiangfeiHan,YongjunWang
Journal:Bioconjugatechemistry(2016):1360--1372
