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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:82kDa
Genbank#:NM_133646
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
ZAKisamemberoftheMAPKKKfamilyofsignaltransductionmoleculesandmediatesgammarADIationsignalingleadingtocellcyclearrest.TheactivityofZAKplaysaroleincellcyclecheckpointregulationaswellasbeinginvolvedinregulatingactinorganization(1).Expressionofkinase-deadZAKinmousefibroblastsdisruptsactinstressfibersandcausesmorphologicchanges.ZAKcanactivateJNKthroughMKK4/MKK7andERK5/p38-gammaviaMKK3/MKK6.ExpressionofZAKincreasesthepopulationofcellsintheG2/Mphaseofthecellcycle,whereasdominant-negativeZAKattenuatedtheG2arrestcausedbygammaradiation(2).
Description:
RecombinanthumanZAK,fulllength,withN-terminalGST-tag,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
Synonym(s):
AZK,MLT,MRK,MLK7,MLTK,mlklak
Purity:
≥75%
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.YangJ-J,etal:MixedlineagekinaseZAKutilizingMKK7andnotMKK4toactivatethec-JunN-terminalkinaseandplayingaroleinthecellarrest.Biochem.Biophys.Res.Commun.297:105-110,2002.
2.GrossE.A,etal:MRK,amixedlineagekinase-relatedmoleculethatplaysaroleingamma-radiation-inducedcellcyclearrest.J.Biol.Chem.277:13873-13882,2002
2.GrossE.A,etal:MRK,amixedlineagekinase-relatedmoleculethatplaysaroleingamma-radiation-inducedcellcyclearrest.J.Biol.Chem.277:13873-13882,2002
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
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