品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / BPS Bioscience / Bpsbioscience/VRK1, Active Human Recombinant Protein, GST-tag/40397/10 µg
商品描述
Species:human
HostSpecies:baculovirus
MW:71kDa
Genbank#:NM_003384
Tag(s):N-terminalGST-tag
a.a:fulllength
Description:
Humanvacciniarelatedkinase1(VRK1),GenBankAccessionNo.NM_003384,fulllengthwithN-terminalGSTtag,MW=71kDa,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
SpecificActivity:
≥2pmol/min/µg
AssayConditions:
5mMMOPS,2.5mMbeta-glycerophosphate,5mMMgCl2,1mMEGTA,0.4mMEDTA,0.05mMDTT,2mMATP.IncubateVRK1with0.25mg/mldephosphorylatedcaseinassubstrateand[33P]-ATPat30&Deg;Cfor15minutes,thenspotreactiononphosphocellulosepaper,fixin1%phosphoricacid,andassaywithascintillationcounter.
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,10mMglutathione,0.1mMEDTA,0.1mMPMSF,25%glycerol,and0.25mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
Storeat-80°C.Stablefor>6monthswhenstoredasdirected.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Nezu,J.,etal.,Genomics1997;45:327-331.
2.Renbaum,P.,etal.,Am.J.Hum.Genet.2009;85:281-289.
2.Renbaum,P.,etal.,Am.J.Hum.Genet.2009;85:281-289.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
