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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
Background:
L-tryptophan(L-Trp)isanessentialaminoacidnecessaryforproteinsynthesisinmammaliancellsandtheL-Trptokynurenine(Kyn)pathwayisfirmlyestablishedasakeyregulatorofinnateandadaptiveimmunity.CatabolismofL-TrptoKynmaintainsanimmunosuppressivemicroenvironmentbystarvingimmunecellsofL-TrpandreleasingdegradationproductsofL-Trpthathaveimmunosuppressivefunctions.Tryptophan2,3dioxygenase(TDO)isupregulatedinmanytumors,providingcancercellswithanavenueforimmuneevasion.
Description:
TheTDOInhibitorScreeningAssayKitisdesignedtomeasureTDOenzymeinhibition.Thekitcomesinaconvenientformat,withenoughreactionsolutionandenzymetoperformatotalof100reactions.TheTDOInhibitorScreeningAssayKitissimpletouse.InhibitorandenzymeareaddedtoasamplecontainingL-Trpsubstrate.Afteraroomtemperatureincubationactivityismeasuredbymeasuringtheabsorptionofreactionproductat&lamBDa; =320-325nm.
Synonym(s):
TDO2,tryptophan2,3dioxygenase
Format:
COMPONENTS:
Instructionsforuse:
Seeproductdatasheetfordetailedprotocol.
Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
UsefulforthestudyofTDOenzymology,screeninginhibitorsandselectivityprofiling.
Reference(s):
Li,J.S.,etal.ArchInsectBiochemPhysiol.2007Feb;64(2):74–87
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
Spectrophotometercapableofmeasuringabsorbanceat=320-325nm.
Warning(s):
DMSO>0.5%,strongacidsorbases,ionicdetergents,highsalt
Avoidfreeze/thawcycles.
ScientificCategory:
Immunotherapy,Metabolicenzyme
ProductType:
AssayKit
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