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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:209kDa(complex)
Genbank#:U79143(p110α),XM_043865(p85α)
Tag(s):N-terminalFLAG-tag(p110α),notagonp85α
a.a:fulllength
Description:
ComplexofN-terminalFLAG-taggedrecombinantfull-lengthhumanp110α(GenBankAccessionNo.U79143),andrecombinantfulllength,humanp85α(notag)(GenBankAccessionNo.XM_043865).CoexpressedinaBaculovirus-infectedSf9cellexpressionsystem.p110αMW=125kDa,p85αMW=84kDa.
Synonym(s):
PIK3CA,p110alpha,Phosphoinositide-3-Kinase,Catalytic,Alpha,PIK3R1,Phosphoinositide-3-Kinase,RegulatorySubunit1(Alpha),GRB1,p85alpha
SpecificActivity:
1500pmol/min/μg.
UnitDefinition:
Aunitofp110α/p85αkinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofPhosphoinositol-3,4,5-Trisphosphate/minat37°C.
Formulation:
25mMTris,pH8.0,69mMNaCl,1.35mMKCl,0.025%Tween-20,3mMDTT,and50%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Vogt,PKetal.,Virology.344(1):131-(2006)
2.Bader,AGetal.,NatRevCancer.Dec5(12):921-9(2005)
2.Bader,AGetal.,NatRevCancer.Dec5(12):921-9(2005)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
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