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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:89kDa
Genbank#:NM_002613
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
PDK1(3-phosphoinositide-dependentproteinkinase)isactivatedbythepresenceofPtdIns(3,4,5)P3orPtdIns(3,4)P2.PDK1thenactivatesproteinkinaseB(PKB)which,inturn,inactivatesglycogensynthasekinase-3(GSK3).ThephosphorylationofotherproteinsbyPKBandGSK3islikelytomediatemanyoftheintracellularactionsofinsulin.Thus,PDK1playsakeyroleinmediatingmanyoftheactionsofthesecondmessenger(s)PtdIns(3,4,5)P3and/orPtdIns(3,4)P2.ThehumanPDK1isa556-residuemonomericenzymecomprisingofacatalyticdomainthatismostsimilartothePKA,PKBandPKCsubfamilyofproteinkinases.
Description:
Human3-phosphoinositide-dependentproteinkinase(PDK1),GenBankAccessionNo.NM_002613,fulllengthwithN-terminalGST-tag,MW=89kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
Synonym(s):
PRO0461;PDPK1;MGC20087;MGC35290;PkB-like;PkB-like1,
SpecificActivity:
≥100 pmol/min/µg
AssayConditions:
AssaywasdoneinKinasebuffercontaining0.2mMDTTusingPDKtidesubstrate(0.1mg/ml)and20μMATP.Reactionwasdoneat30˚Cfor45min.AmountofATPtransferredwasmeasuredusingKinase-Glo®reagent(Promega)
Formulation:
40mMTrispH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,3mMDTTand20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Yang,KJ.,etal.,JBiolChem.2008Jan18,283(3):1480-91.
2.Kikani,CK.,etal.,JCellBiochem.2005Dec15,96(6):1157-62.
ApplicationReference:
PDK1controlsupstreamPI3KexpressionandPIP3generation(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
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