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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:74kDa
Genbank#:NM_017572
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
MNK2(MAPkinase-interactingkinase2)containsaconservedC-terminalERK-interactingdomain,acatalyticdomainwithhomologytothecalcium/calmodulin-dependentfamilyofkinases,andputativeMAPkinasephosphorylationsiteslocatedwithintheTloopofthekinasedomain.MNK2bindstightlytothegrowthfactor-regulatedMAPkinases,ERK1andERK2.ERKandp38phosphorylateMNK2,whichstimulatesitsinvitrokinaseactivitytowardasubstrate,eukaryoticinitiationfactor-4E(eIF-4E)(1).Ayeasttwo-hybridscreenshowedtheMnk2proteininteractedwiththeligand-bindingdomainofestrogenreceptorbeta(ERbeta)(2).
Description:
RecombinanthumanMNK2(MAPkinaseinteractingserine/threoninekinase2),alsoknownasGPRK7(Gprotein-coupledreceptorkinase7),GenBankAccessionNo.NM_017572,fulllength,withN-terminalGSTtag,MW~74kDa,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
Synonym(s):
MKNK2;GPRK7
Purity:
≥75%
SpecificActivity:
84pmol/min/µg
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Waskiewicz,AJ.etal:Mitogen-activatedproteinkinasesactivatetheserine/threoninekinasesMnk1andMnk2.EMBOJ.1997Apr15;16(8):1909-20.
2.Slentz-Kesler,K.etal:IdentificationofthehumanMnk2gene(MKNK2)throughproteininteractionwithestrogenreceptorbeta.Genomics.2000Oct1;69(1):63-71.
2.Slentz-Kesler,K.etal:IdentificationofthehumanMnk2gene(MKNK2)throughproteininteractionwithestrogenreceptorbeta.Genomics.2000Oct1;69(1):63-71.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
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