TNFα(Sigma,#T0157-10UG)
PMA(LCLaboratories,#P1680)
Ionomycin(Sigma,#I3909)
AssayMedium:ThawMedium2(BPSCat.#60184)
96-welltissueculturetreatedwhiteclear-bottomassayplate(Corning#3610)
ONE-Stepluciferaseassaysystem(BPSCat.#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity
Luminometer
2.MoriN.et.al.(2002)Bay11-7082inhibitstranscriptionfactorNF-kBandinducesapoptosisofHTLV-1-infectedTcelllinesandprimaryadultT-cellleukemiacells.Blood.100:1828-1834
3.TakadaY,KobayashiY,AggarawalBB(2005)EvodiamineabolishesconstitutiveandinducIBLeNF-kappaBactivationbyinhibitingIkappaBalphakinaseactivation,therebysuppressingNF-kappaB-regulatedantiapoptoticandmetastaticgeneexpression,up-regulatingapopotissandinhibitinginvasion.JBiolChem.280:17203
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