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商品描述
Species:Human
HostSpecies:FreeStyle293-Fcells
MW:66kDa
Genbank#:NM_199141
Tag(s):N-terminalFLAG-tag
a.a:2–end
Background:
CARM1(PRMT4)methylates(mono-andasymmetricdimethylation)thearginineresiduesinseveralproteinsinvolvedinDNApackaging,transcriptionregulation,pre-mRNAsplicing,andmRNAstABIlity.ItisrecruitedtopromotersupongeneactivationtogetherwithhistoneacetyltransferasesfromEP300/P300andp160families,methylateshistoneH3atArg17(H3R17me),formingmainlyasymmetricdimethylarginine(H3R17me2a),resultingintranscriptionactivationviachromatinremodeling.CARMalsomethylatesargininesinseveralotherproteins,includingEP300/P300atArg-2142,Arg-580,andArg-604.CARM1actsascoactivatorforPPARG,andseemstobeinvolvedinp53/TP53transcriptionalactivation.
Description:
HumanPRMT4(CARM1),GenBankAccessionNo.NM_199141,a.a.2-end,withN-terminalFLAG-tag,MW=66kDa,expressedinFreeStyle293-Fcells.
UniProt
Q86X55
Synonym(s):
PRMT4,CARM1,Coactivator-AssociatedArginineMethyltransferase1
AssayConditions:
50µlreactionmix(1xHMTAssayBuffer5,BPScatalog#52191and5-1000ngmethyltransferasePRMT4)isaddedtowellscoatedwiththesubstrateonaThermoHBCNeutravidinblackplate.Incubateatroomtemperaturefor1hr.AddantibodyagainstmethylatedR3residueofhistoneH4,incubate1hr.AddsecondaryHRP-labeledantibodyandincubate30min.Finally,addHRPchemiluminescentsubstratesandreadluminesence.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and80ng/μlFLAGpeptide
Format:
Aqueousbuffersolution
Storage/Stability:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.LiH.etal.,J.Biol.Chem.277(47),44623-44630(2002).
2.FrietzeS.etal.,CancerRes.68(1),301-306(2008).
ApplicationReference(s):
1. TransitionstatemimicsarevaluablemechaNISTicprobesforstructuralstudieswiththeargininemethyltransferaseCARM1(2017)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyltransferase
ProductType:
RecombinantProtein
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