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商品描述
Species:Human
HostSpecies:E.coli
MW:68kDa
Genbank#:NM_001536
Tag(s):N-terminalGST-tag
a.a:2–end
Background:
PRMT1isaproteinargininemethyltransferasethatfunctionsasahistonemethyltransferasespecificforhistoneH4.PRMT1specificallymethylatesarginine-3ofhistoneH4invitroandinvivo.Methylationofarg3byPRMT1facilitatessubsequentacetylationofhistoneH4tailsbyp300.PRMT1performsmethylationofarg31ofSTAT1andthisisrequiredfortranscriptioninducedbyIFN-alpha/IFN-beta.ArgininemethylationofSTAT1isanadditionalposttranslationalmodificationregulatingtranscriptionfactorfunction,andalterationofargininemethylationmightberesponsIBLeforthelackofinterferonresponsivenessobservedinmanymalignancies.TypeIPRMTs((CARM1,PRMT1,PRMT2,PRMT3,PRMT6,andPRMT8)catalyzeasymmetricdimethylarginine(ADMA),whiletypeIIPRMTs(e.g.PRMTs5and7)catalyzeSDMA.PRMT1istheprimarymethyltransferasethatdepositstheADMAmark,anditaccountsforover90%ofthistypeofmethylation.
Description:
HumanPRMT1(GenBankAccessionNo.NM_001536),a.a.2-end,withN-terminalGST-tag,MW=68kDa,expressedinanE.coliexpressionsystem.
UniProt
Q99873
Synonym(s):
PRMT1,HRMT1L2,IR1B4,HCP1
SpecificActivity:
0.52pmol/min/µg
AssayConditions:
50µlreactionmix(20mMphosphatebufferpH7.4,20µMS-adenosylmethionine,and1-5ngmethyltransferasePRMT1)addtothewellscoatedwiththesubstrate.Incubateatroomtemperaturefor1hr.AddantibodyagainstmethylatedR3residueofhistoneH4,incubate1hr.AddsecondaryHRP-labeledantibodyandincubate30min.Finally,addHRPchemiluminescentsubstratesandreadluminesence.
Formulation:
40mMTrispH8.0,110mMNaCl,2.2mMKCl,0.04%Tween,20%glycerol,3mMDTT
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.LeRomancer,M.etal.,Mol.Cell31,2,212-221,2008.
2.Fronz,Ketal.,J.Biol.Chem.283,29,20408-20420,2008.
ApplicationReference(s):
1.ASensitiveLuminescentAssayfortheHistoneMethyltransferaseNSD1andOtherSAM-DependentEnzymes(2014)
2.Selectiveinhibitorsofbacterialt-RNA-(N(1)G37)methyltransferase(TrmD)thatdemonstratenovelorderingoftheliddomain(2013)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyltransferase
ProductType:
RecombinantProtein
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