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商品描述
Species:Mouse
HostSpecies:E.coli
MW:16kDa
Genbank#:P61148
a.a:16–155
Background:
aFGFandbFGFdisplay55%homologywhichislimited,however,toafewfunctionaldomains.ThestructureofaFGF,revealedbyX-raycrystallography,showsasimilarfoldingstructureasIL-1.BecauseaFGFbindstothesamereceptorasbFGF,aFGFdisplaysmoreorlessthesamespectrumofactivities.Itis,however,generallylessactivethanbFGFinsimilarassays.FGFreceptorsareencodedbyagenefamilyconsistingofatleastfourreceptortyrosinekinasesthattransducesignalsimportantinavarietyofdevelopmentalandphysiologicalprocessesrelatedtocellgrowthanddifferentiation.
Description:
RecombinantmurineaFGF(acidicFibroblastGrowthFactor)isadisulfide-linkedmonomerproteinconsistingof141a.a.andmigratesasanapproximately16kDaproteinunderreducingandnon-reducingconditions.NativemurineaFGF,generatedbytheproteolyticremovalofthesignalpeptideandpropeptide,hasacalculatedmassof16kDa.OptimizedDNAsequenceencodingmurineacidicFibroblastGrowthFactormaturechainwasexpressedinE.coli.
UniProt
P61148
Synonym(s):
aFGF,acidicFibroblastGrowthFactor,Heparin-BindingGrowthFactor1,HBGF-1,FGF-1,FibroblastGrowthFactor-1
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BIOLOGicalActivity:
TheED50asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGFreceptorsis≤10ng/ml,correspondingtoaspecificactivityof≥1x105units/mg.
Formulation:
Lyophilizedfrom0.2µmfilteredPBSsolution.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.Expressionofacidicfibroblastgrowthfactor(aFGF)andfibroblastgrowthfactorreceptor4(fgfr4)inbreastfibroadenomas.J.Clin.Pathol.,Jan2001,54:37-41.
2.Requirementofphosphatidylinositol3-kinaseactivityfortranslocationofexogenousaFGFtothecytosolandnucleus.J.Biol.Chem.,Apr2000,275:11972.
2.Requirementofphosphatidylinositol3-kinaseactivityfortranslocationofexogenousaFGFtothecytosolandnucleus.J.Biol.Chem.,Apr2000,275:11972.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
FNLPLGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESAGEVYIKGTETGQYLAMDTEGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
