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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:67kDa
Genbank#:NM_016614
Tag(s):N-terminalHis-GST-tags
a.a:fulllength
Description:
HumantyrosylDNAphosphodiesterase2,orTDP2,(GenBankAccessionNo.NM_016614),fulllengthwithN-terminalHis-GST-tag,MW=67kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
Synonym(s):
PDE,tyrosylDNAphosphodiesterase2
SpecificActivity:
≥186pmol/min/µg
AssayConditions:
Enzymereactionwasperformedinareactionbuffer(200µl)containing50mMTris(pH7.4),1mMMgCl2,50mMKCl,1mMDTT,0.1mg/mlBSA,2mMT5PNPand600ngTDP2.Reactionwascontinuouslymonitoredat415nmatroomtemperaturefor20min.Specificactivitywascalculatedbasedonthemolarextinctioncoefficientoftheproduct(p-nitrophenol),e=15,000M-1cm-1
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,200mMimidazole,20%glyceroland3mMDTT
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Reference(s):
1.Loughney,K,etal.,Int.J.Impot.Res.2005,17(4):320-5
2.Weeks,JL,etal.,Int.J.Impot.Res.2005,17(1):5-9
2.Weeks,JL,etal.,Int.J.Impot.Res.2005,17(1):5-9
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
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