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商品描述
Species: Human
Host Species: E. coli
MW: 62 kDa
Genbank #: NM_133330
Tag(s): N-terminal GST-tag
a.a: 941–1240
Background:
NSD2 is a histone methyltransferase that preferentially methylates Lys27 of histone H3. Isoform RE-IIBP may act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment. Mutations in the gene for NSD2 are linked to Wolf-Hirschhorn syndrome (WHS), a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4, and is also associated with chromosomal translocations in multiple myelomas.
Description:
Human NSD2, also known as RE-IIBP, MMSET, and WHSC1 (GenBank Accession No. NM_133330), a.a. 941-1240 (catalytic domain) with N-terminal GST-tag, MW = 62 kDa, expressed in E. coli.
UniProt
O96028
Synonym(s):
WHSC1, Wolf-Hirschhorn Syndrome Candidate 1, MMSET, Multiple Myeloma SET Domain Containing Protein, NSD2, Nuclear SET Domain-Containing Protein 2, REIIBP, WHS, TRX5
Specific Activity:
0.036 pmol/min/µg
Assay Conditions:
Assay was performed using the BPS NSD2 Chemiluminescent Assay Kit (Cat. #53009) according to recommended protocol, with 5 µM S-adenosylmethionine on biotinylated H3 peptide-coated plates. Enzyme reaction was performed for 2 hours at room temperature.
Formulation:
50 mM Tris-HCl, pH 8.5, 300 mM NaCl, 5 mM DTT, 10 mM glutathione, and 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Kassambara A. et al., Biochem. Biophys. Res. Commun. 379 (4), 840-845 (2009).
2. Brito J.L. et al., Haematologica 94 (1), 78-86 (2009).
2. Brito J.L. et al., Haematologica 94 (1), 78-86 (2009).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
