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商品描述
Species: Human
Host Species: HEK293
Description:
Nucleosomes prepared from
mammalian HEK293 cells as a substrate for
histone methyltransferase assay.
Formulation:
10 mM Tris-HCl, pH 7.2, 5
mM NaCl, 1 mM MgCl2, 0.5 mM CaCl2, 125
mM EDTA, and 10 mM Sodium Butyrate
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.
Reference(s):
1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11.
2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.
2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.
Notes:
The concentration of this product is determined by Bradford and represents only the protein concentration.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase/Substrate
Product Type:
Purified Protein
Data shown is lot-specific. Contact us for specific information on other lots.