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商品描述
Species:Human
HostSpecies:E.coli
MW:65kDa
Genbank#:NM_016539
Tag(s):N-terminalGST-tag
a.a:fulllength
Description:
HumanSirtuin6(GenBankAccessionNo.NM_016539),fulllengthwithN-terminalGST-tag,MW=65kDa,expressedinanE.coliexpressionsystem.
UniProt
Q8N6T7
Synonym(s):
Sirtuin6,SIRT6
SpecificActivity:
1.5pmol/min/µg
AssayConditions:
25mMTris/HCl,pH8.0,137mMNaCl,2.7mMKCl,1mMMgCl2,and0.1mg/mlBSA,20µMBPSSIRT6substrate2(Catalog#50084),500µMNAD+andSirtuin6(18–2000ng).Incubationcondition:60minat37ºCfollowedbydevelopingfor15minatroomtemperature.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat–80°C.Avoidfreeze/thawcycles.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Kanfi,Y.etal.,FEBSLett.2008Mar5;582(5):543-8.
2.Mostoslavsky,R.etal.,Cell.2006Jan27;124(2):315-29.
2.Mostoslavsky,R.etal.,Cell.2006Jan27;124(2):315-29.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Deacetylase
ProductType:
RecombinantProtein
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