品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:88kDa
Genbank#:NM_005153
Tag(s):N-terminalFLAG-tag
a.a:2–798(end)
Description:
HumanUSP10(Ubiquitin-SpecificProtease10)orUbiquitinC-terminalHydrolase10,GenBankAccessionNo.NM_005153,a.a.2-798(end)withanN-terminalFLAG-tag,MW=88kDa,expressedinabaculovirus-infectedSf9cellexpressionsystem.
UniProt
Q14694
Synonym(s):
USP10,Ubiquitin-SpecificProtease10,UbiquitinC-terminalHydrolase10,Ubch10,USP-10
SpecificActivity:
1.5pmole/min/µg
AssayConditions:
Reactionwasperformedin50mMTris,pH7.4,1mMDTT,0.5mMEDTA,500nMUb-AMC,andUSP10ina50µLvolume.Reactionwasincubatedat37°Cfor15min.andfluorescentsignalwasmeasuredatexcitation=340nm,emission=460nm.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,90?g/mlFLAGpeptide,10%glycerol,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Faus,H.,etal.(2005).Mol.Cell.Endocrinol.245(1-2):138-46.
2.Soncini,C.,etal.(2001).Oncogene20(29):3869-79.
3.Yuan,J.,etal.(2010).Cell140(3):384-96.
ApplicationReference:
ResveratrolinducesapoptosisbydirectlytargetingRas-GTPase-activatingproteinSH3domain-bindingprotein1(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Ubiquitins:Deubiquitinase(DUB)
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
