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商品描述
Species:Human
MW:8722Da
Genbank#:P62987(ubiquitin)
Tag(s):
Background:
Ubiquitin-AMCispreparedviatheconjugationoftheC-terminusofmature,Ubiquitinwith7-amino-4-methylcoumarin(AMC).ThisconjugationquenchestheintrinsicfluorescenceofAMC.ThehighpurityofBPS"sUbiquitin-AMCcomparedtothatofothercommercialvendorsleadstoagreatersignal:background.
Description:
Ubiquitin-AMCisafluorogenicsubstrateforubiquitinhydrolasesbasedontheC-terminusderivatizationofubiquitinwith7-amido-4-methylcoumarin(AMC).UponincubationwithaproteaserecognizingUbiquitin,suchasUSP2orUCHL3,AMCisreleasedandtheincreaseinfluorescencecanbemeasuredusingEx380nmandEm460nmwavelengths.Thisproteincontainsnoextraneoustags.
UniProt
P09936
Synonym(s):
Ub-AMC
Purity:
≥95%byHPLC.
BIOLOGicalActivity:
TypicalenzymeconcentrationsforUCH-L3are10-100pMandforIsopeptidase-Tare10-100nM.
AssayConditions:
ReleaseofAMCfluorescencebyUCHenzymescanbemonitoredusingEx380nmandEm460nmwavelengths,respectively.
Solubility:
SolubleinDMSOoraqueousbuffers(>1mg/ml)
Format:
Lyophilizedsolid
Storage/StABIlity:
Storeinthedarkatorbelow–80°C.Stableassuppliedforupto1yearwhenstoreddessicatedat–80°C.StoreDMSOsolutionsat–80°Cforupto1month.Avoidfreeze/thawcyclesofsolutions.
Application(s):
Usefulasasubstratefordeubiquitinatingenzymes(DUBs),includingubiquitinC-terminalhydrolases(UCHs)andubiquitinspecificproteases(USPs),aswellasinvestigationofdeconjugatingenzymesubstratespecificityandforscreeningformodulatorsof
Reference(s):
1.Dang,L.C.,etal.KineticandmechaNISTicstudiesonthehydrolysisofubiquitinC-terminal7-amido-4-methylcoumarinbydeubiquitinatingenzymes.Biochemistry,37,1868-1879(1998).
2.Mason,D.E.,etal.Substrateprofilingofdeubiquitinhydrolaseswithapositionalscanninglibraryandmassspectrometry.Biochemistry,43,6535-44(2004).
2.Mason,D.E.,etal.Substrateprofilingofdeubiquitinhydrolaseswithapositionalscanninglibraryandmassspectrometry.Biochemistry,43,6535-44(2004).
Warning(s):
Protectfromlight
AminoAcidSequence:
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
ScientificCategory:
UbiquitinationSubstrate
ProductType:
Substrate
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