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商品描述
Species: Human
Host Species: E. coli
MW: 21 kDa
Genbank #: NM_007019
Tag(s): N-Terminal His-Tag
Background:
UBE2C or ubiquitin-conjugating enzyme E2C is a member of the E2 ubiquitin-conjugating enzyme family which is required for the destruction of mitotic cyclins and for cell cycle progression. UBE2C acted as a dominant-negative inhibitor which blocks the destruction of mitotic cyclins A and B and the onset of anaphase in frog embryos and mammalian cells. High UBE2C mRNA expression is associated with poor disease-free survival and overall survival. High tumor grade, as well as high Ki67 protein expression, was more frequent in the high-expression group of UBE2C.
Description:
Recombinant full-length Human UBE2C, also known as dJ447F3.2, and UBCH10. Genbank Accession No. NM_007019, with an N-terminal His-tag, expressed in E. Coli cells via a baculovirus expression system. MW=21 kDa.
Synonym(s):
dJ447F3.2, UBCH10
Formulation:
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, and 25% glycerol.
Storage / Stability:
At least 6 months at –70°C. Avoid freeze/thaw cycles.
Application(s):
Useful for the study of ubiquitin enzyme kinetics, and as a positive control for Western blot studies.
Reference(s):
1. Mondal, S., et al., Anal. Biochem. 2016; 510: 41-51.
2. Rape, M., et al., Nature. 2004; 432: 588-595.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Ubiquitination
Product Type:
Activating Enzyme
Data shown is lot-specific. Contact us for specific information on other lots.
