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商品描述
Species: Human
Host Species: HEK293
MW: 72 kDa
Genbank #: NM_004530
Tag(s): C-terminal His-tag
a.a: 30 - 660 (end)
Description:
Human matrix metallo-peptidase-2 (MMP2), also known as gelatinase A (GenBank Accession No. NM_004530), a.a. 30 - 660 (end), with C-terminal His-tag, MW=72 kDa, expressed in an HEK293 cell expression system.
UniProt
P08253
Synonym(s):
matrix metallo-peptidase-2, MMP2, gelatinase A, MMP
Specific Activity:
≥ 511 pmole/min/µg
Assay Conditions:
Activation: This is a proform enzyme and
requires activation prior to testing activity.
Dilute the enzyme to 100 ng/ml in a solution
containing 50 mM HEPES, pH 7.4, 10 mM
CaCl2, 0.05% Brij-35, and 1 mM APMA
(amino-phenyl mercuric acetate). Incubate at
37ºC for 2 hours.
Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1 (Anaspec). Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393.
Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1 (Anaspec). Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Van Dijk, M., et al.,Front Genet.
2012; 3:22.
2. Klotzsche-von Ameln, A., et al.,
Cancer Biol Ther. 2012 Feb 15;
13(4): 216 - 223.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protease
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
