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商品描述
Species: Human
Host Species: E. coli
MW: 52.5kDa
Genbank #: NM_152615, var2
Tag(s): N-terminal GST-tag
a.a: 221–444(end)
Description:
Human poly (ADP-ribose) polymerase family, member 15 (PARP15) (GenBank Accession No. NM_152615, var.2), a.a. 221-444(end) with N-terminal GST-tag, MW = 52.5 kDa, expressed in an E. coli expression system.
UniProt
Q460N3
Synonym(s):
poly (ADP-ribose) polymerase 15, poly(ADP-ribosyl)transferase 15, PARP15
Unit Definition:
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Assay Conditions:
Enzyme reaction is conducted using
BPS's PARP15 Assay Kit (Catalog #80567) at room temperature for 30 minutes.
Formulation:
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Andersson CD., et al. J. Med. Chem. 2012 Sep 13;55(17):7706-18.
2. Ricardo C. T. Aguiar., et al. J. Biochem.2005 Oct 280;(40):33756–65.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PARP
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
