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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:66.5kDa
Genbank#:NM_017554
Tag(s):N-terminalGST-tag
a.a:1470–1801(end)
Description:
HumanPARP14(GenBankAccessionNo.NM_017554),a.a.1470-1801(end)withN-terminalHis-GST-tag,MW=66.5kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q460N5
Synonym(s):
poly(ADP-ribose)polymerase14,poly(ADP-ribosyl)transferase14,PARP14
UnitDefinition:
OneunitofPARPincorporates100pmolesofpoly(ADP)in1minutefromNADintotheacid-insolubleform.
AssayConditions:
EnzymereactionisconductedusingBPS"sPARP14AssayKit(Catalog#80568)atroomtemperaturefor1hour.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,188mMimidazole,0.04%Tween-20,3mMDTT,20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Barbarulo,A.,etal.,Oncogene.10(1038),448(2012).
2.Cho,SH.,etal.,ProcNatlAcadSciUSA.108(38),15972-7(2011).
ProductSpecificReference(s):
1.Wang,P.,etal.,Tetrahedron.70(35), 5666–5673(2014).
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PARP
ProductType:
RecombinantProtein
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