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商品描述
Species:Human
HostSpecies:E.coli
MW:14.6kDa
Genbank#:NM_003528
Tag(s):N-terminalHis-tag
a.a:2–126(end)
Description:
HumanHistone2B,GenBankAccessionNo.NM_003528,a.a.2-126(end)withN-terminalHis-tag,MW=14.6kDa,expressedinanE.coliexpressionsystem.
UniProt
Q16778
Synonym(s):
H2B/c,H2BFC,HIST1H2BL,HistoneH2B
Formulation:
50mMTris-HCl,pH8.0,150mMNaCl,10%glycerol,1mMPMSFand1mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulasasubstrateforhistonemethyltransferaseandacetyltransferaseassays.IdealforscreeningsmallmolecularinhibitorsofhistonemodifyingenzymesfordrugdiscoveryandHTSapplications.
Reference(s):
1. Osley, M.A., Brief Funct. Genomic Proteomic.5(3):179-89(2006).
2. Wyrick, J.J. and Parra, M.A., Biochim BiophysActa.1789(1):37-44(2009).
3. Emre,N.C. andBerger,S.L.,ColdSpring Harb Symp Quant Biol. 69: 289-99(2004).
ApplicationReference(s):
Differentialmodeofantimicrobialactionsofarginine-richandlysine-richhistonesagainstGram-positiveStaphylococcusaureus(2013)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyltransferase/Substrate
ProductType:
RecombinantProtein
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