快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / BPS Bioscience / Bpsbioscience/rPDE7A-HEK293 Recombinant Cell Line/60408/2 vials

Bpsbioscience/rPDE7A-HEK293 Recombinant Cell Line/60408/2 vials

价格
￥165000.00
货号：60408
浏览量：127
品牌：BPS Bioscience
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Species:Rat
HostSpecies:HEK293(human)
Genbank#:NM_031080
Background:
Phosphodiesterases(PDEs)regulatetheintracellularlevelsofcAMPandcGMPbyhydrolyzingcAMPandcGMPtotheirinactive5"monophosphates.Thesecyclicnucleotidesplayanimportantroleassecondmessengersindiversephysiologicalfunctions.PDE7isacAMP-specificenzymeandtwoPDEgenes(PDE7AandPDE7B)havebeenidentified.PDE7iswidelyexpressedinvarioustissues,withPDE7Afoundprimarilyinskeletalmuscle,Tlymphocytes,andpancreas,whilehighlevelsofPDE7Baredetectedinbrain,heart,andliver.InhibitionofPDE7activitybyitsinhibitorsleadstoelevatedintracellularlevelofcAMP.
Description:
RecombinantHEK293celllineexpressingratPDE7A(phosphodiesterase7A,accessionnumberNM_031080).

N-terminalFLAG-taggedratPDE7Ahasbeenstablyexpressedinahumanembryonickidney(HEK293)celllineanditsexpressionwasconfirmedbyWesternblotting.TheregulationofintracellularlevelofcAMPbyratPDE7AinratPDE7Astably-expressedHEK293cellswascharacterizedbyacell-basedreporterassayusingpCRE-lucreportervector.pCRE-luccontainsaluciferasegenethatisunderthecontrolofthecAMPresponseelement(CRE).WhencellstransientlytransfectedwithpCRE-lucreporterwereactivatedbyforskolin,thelevelofcAMPwasupregulatedinparentalHEK293cellsinducingtheexpressionofluciferasereporter,whereasratPDE7A-HEK293cellsshowedreductioninthelevelofcAMP,resultinginloweredlevelsofluciferaseexpression.InhibitionofPDE7AactivitybyBRL50481,aPDE7Ainhibitor,restoredthecAMPlevel,resultinginhigherluciferaseactivity.
UniProt
O08593
Synonym(s):
PDE7A,phosphodiesterase7A
SuppliedAs:
Eachvialcontains~1X106cellsin1mlof10%DMSO.
MycoplasmaTesting:
ThecelllinehasbeenscreenedusingthePCR-basedVenorGeMMycoplasmaDetectionkit(SigmaAldrich)toconfirmtheabsenceofMycoplasmaspecies.
Format:
aqueoussolutioncontainingDMSO
Instructionsforuse:
Seedatasheetfordetailedculturingandassayprotocol.
Storage/StABIlity:

Storeinliquidnitrogenimmediatelyuponreceipt.

Application(s):
UsefultomonitorcAMPandPDE7AsignalingpathwayactivityandtoscreenactivatorsorinhibitorsofPDE7A.
Reference(s):
1.Malik,R.etal.(2008)Appl.Microbiol.Biotechnol.77(5):1167-1173.
2.FanChung,K.(2006).Eur.J.Pharmacol.533(1-3):110-117.
Notes:

Contactusforpricing.

LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.

Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
PDE
ProductType:
CellLine
Datashownislot-specific.Contactusforspecificinformationonotherlots.
BPS Bioscience生物素化，生物素标记通常用于蛋白质和其他目标分子的非放射性标记和纯化。生物素标记利用生物素（维生素H）与抗生物素蛋白或抗生蛋白链菌素之间异常强的相互作用。生物素与抗生物素蛋白或抗生蛋白链菌素的亲和力是已知的蛋白质和配体最强的非共价相互作用之一，在10 -15附近表现出解离常数（K d）。此外，这种相互作用可抵抗高或低pH，高盐浓度，极端温度和变性剂。生物素-亲和素相互作用的这些性质使其可用于纯化和/或检测目的蛋白质。BPS具有许多蛋白质，这些蛋白质已通过化学方法通过各种反应基团以及使用AviTag TM  技术进行酶促生物素标记。BPS的生物素化产品组合包括用于标记伯胺和仲胺，炔基，硫醇，羧基和磷酸基以及各种PEG基团的试剂。这种灵活性使研究人员能够满足他们所有的生物素化需求。