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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:111kDa
Genbank#:NM_001165899
Tag(s):N-terminalGST-tag
a.a:2–748(end)
Description:
HumanPDE4D7(GenBankAccessionNo.NM_001165899),a.a.2-748(end)withN-terminalGST-tag,MW=111kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q08499
Synonym(s):
PDE4D7,phosphodiesterase4D7
SpecificActivity:
≥10,000pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cAMPto5"-AMPperminuteat37°C.
AssayConditions:
10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcAMP,2.5kU5"-nucleotidase,andserialdilutionsofPDE4D7at37°Cfor20min.Quantifiedby5"-nucleotidasecleavingthe5"-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,3mMDTT,20mMGlutathioneand10%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Wang,D.,etal.,(2003).CellSignal.15(9):883-91.
2.Houslay,M.D.(2005).MolPharmacol.(2005).68(3):563-7
2.Houslay,M.D.(2005).MolPharmacol.(2005).68(3):563-7
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
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