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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:117kDa
Genbank#:NM_001111309
Tag(s):N-terminalGST-tag
a.a:2–825(end)
Description:
HumanPDE4A10,alsoknownasPDE4Aproteinisoform6,transcriptvariant3(GenBankAccessionNo.NM_001111309),a.a.2-825(end)withN-terminalGST-tag,MW=117kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
P27815
Synonym(s):
PDE4Aproteinisoform6,transcriptvariant3,PDE4A10
SpecificActivity:
≥4,400pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cAMPto5"-AMPperminuteat37°C.
AssayConditions:
AssayedusingBPS"sPDE4AAssayKit,Cat.#60340.Assayconditions:10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcAMP,2.5kU5"-nucleotidase,andserialdilutionsofPDE4A10at37°Cfor20min.Quantifiedby5"-nucleotidasecleavingthe5"-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,20%glyceroland3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.McCahill,A.,etal.CellSignal.2008.Nov;20(11):2071-83.
2.McPhee,I.,etal.,CellSignal.2001.Dec;13(12):911-8.
2.McPhee,I.,etal.,CellSignal.2001.Dec;13(12):911-8.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
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