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商品描述
Species:Human
HostSpecies:E.coli
MW:74.6kDa
Genbank#:NM_006709
Tag(s):N-terminalGST-tag
a.a:785–1210
Background:
EHMT2(G9a)isalysine-preferringhistonemethyltransferase.EHMT2canmethylateitselfatK165andthisautomethylationisnecessaryandsufficienttomediateinvivointeractionofEHMT2withheterochromatinprotein-1(HP1)(1).EHMT2isresponsIBLeforthemajorityofdimethylationofhistoneH3atlysine9(H3K9me2)andisrequiredfortheefficientrepressionofdevelopmentallyregulatedgenesduringembryonicstemcelldifferentiation(2).EHMT2isoverexpressedinvariouscancersandcancanspecificallymethylatep53atLys(373).EHMT2polymorphismshavebeenassociatedwithcolorectalcancer.
Description:
HumanG9a,alsoknownasEHMT2(euchromatichistone-lysineN-methyltransferase2)andKMT1C(GenBankAccessionNo.NM_006709),a.a.785-1210,withN-terminalGST-tag,MW=74.6kDa,expressedinanE.coliexpressionsystem.
UniProt
Q96KQ7
Synonym(s):
G9a,EHMT2,KMT1C,euchromatichistone-lysineN-methyltransferase2
SpecificActivity:
22pmol/min/µg
AssayConditions:
50µlreactionmix(50mMTrispH8.8,5mMMgCl2,4mMDTT,20µMS-adenosylmethionineand0-40ngG9a)isaddedtowellscoatedwiththesubstrate.Incubatefor1hour.AddantibodyagainstmethylatedK9residueofhistoneH3,incubateatroomtemperaturefor1hour.Finally,incubate1hourwiththeEuropium-labeledsecondaryantibody.Timeresolvedfluorescencemeasuredatroomtemperature(λexc=330nm,λem=620nm).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and16mMglutathione
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Yuan,X.etal.,Mol.Cell27,4,585-595,2007.
2.Nishida,M.etal.,GenesCells12,7,877-888,2007.
ApplicationReference(s):
Onthehistonelysinemethyltransferaseactivityoffungalmetabolitechaetocin(2013)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyltransferase
ProductType:
RecombinantProtein
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