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商品描述
Species:Human
HostSpecies:E.coli
MW:37kDa
Genbank#:NM_004179
Tag(s):N-terminalHis-tag
a.a:105-420
Description:
Humantryptophan5-hydroxylase1(TPH1)alsoknownasTRPH,GenBankAccessionNo.NM_004179,a.a.105-420withanN-terminalHis-tag,MW=37kDa,expressedinanE.colicellexpressionsystem.
UniProt
P17752
Synonym(s):
tryptophan5-hyrdoxylase,TRPH
AssayConditions:
Reactionwasperformedatroomtemperatureinassaybuffercontaining50mMMOPS,pH7.0,60μMtryptophan,100mMammoniumsulfate,100μMferrousammoniumsulfate,0.5mMTCEP,0.3mM6-methyltetrahydropterin,0.05mg/mlcatalase,1mMDTTandTPH1.FluorescencesignalwasmeasuredatEm360nm/Ex300nm.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,20%glycerol,3mMDTTand170mMimidazole.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Cote,F.,etal.,Proc.Nat.Acad.Sci.USA.2003;100(23):13525-13530.
2.Walther,D.J.,etal.,Science.2003;299:76.
2.Walther,D.J.,etal.,Science.2003;299:76.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
MetabolicEnzyme,Immunotherapy
ProductType:
RecombinantProtein
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