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商品描述
Species:Human
HostSpecies:E.coli
MW:55kDa
Genbank#:NM_004567/NP_004558
Tag(s):N-terminalHistag
a.a:2-469
Description:
Humanrecombinant6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase4(PFKFB4),alsoknownas6PF-2-K/Fru-2,6-P2ase4,andPFK/FBpase4,a.a.2-469withanNterminalHis-tag,GenBankAccessionNo.NM_004567/NM_004558,MW=55kDa,expressedinanE.colicellexpressionsystem.
UniProt
Q16877
Synonym(s):
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase4;6PF-2-K/Fru-2,6-P2ase4;PFK/FBpase4
SpecificActivity:
16pmol/min/μg
AssayConditions:
Thereactionmixturecontained50mMTris-HCl,pH7.5,12.5mMMgCl2,1mM-D-fructose-6-phosphate,20μMATP,2mMDTT,5mMKH2PO4,andvariousamountsofPFKFB4inafinalvolumeof50uL.Thereactionwasincubatedat30°Cfor30min,theamountofATPconsumedwascalculatedusingkinase-gloplusreagent(Promega).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,200mMimidazoleand20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Minchenko,O.H.,etal.,ActaBiochimicaPoloncia.2005;52(4):881-888.
2.Goidts,V.,etal.,Oncogene.2012;31:3235-3243.
2.Goidts,V.,etal.,Oncogene.2012;31:3235-3243.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
MetabolicEnzymes-Kinase
ProductType:
RecombinantProtein
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