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商品描述
Species:Human
HostSpecies:E.coli
MW:82kDa
Genbank#:NM_005746
Tag(s):N-terminalGST-tag
a.a:2–491(end)
Description:
HumanNicotinamidephosphoribosyltransferaseprotein(NAMPT),alsoknownasPBEF1orvisfatin(GenBankAccessionNo.NM_005746)a.a.2-491,withN-terminalGST-tag,MW=82kDa,expressedinanE.colicellexpressionsystem.
UniProt
P43490
Synonym(s):
Nicotinamidephosphoribosyltransferaseprotein,NAMPT,PBEF1,visfatin,pre-B-cellcolony-enhancingfactor1,NMPT
SpecificActivity:
≥0.6pmol/min/µg
AssayConditions:
Thereactionmixturecontained50mMTris-HCl,pH8.0,12.5mMMgCl2,0.1mMnicotinamide,0.4mMPRPP,2mMATP,30µg/mlofalcoholdehydrogenase,10µg/mlofNMNAT(BPS,#71090),1.5%alcohol,1mMDTT,0.02%BSA,andvariousamountofNAMPTinafinalvolumeof50µl.ReactionswereinitiatedwiththeadditionofNAMPT,andtheincreaseinNADHfluorescence(Ex340nm/Em460nm)wascontinuouslymonitoredfor30minatroomtemperature.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,10mMglutathione,0.04%Tween-20,20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofproteinbindingassays,enzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.AmalBetal.,Mol.Cell.Biol.14(2)
2.MartinPR.etal.,J.Bacteriol.183(4)
2.MartinPR.etal.,J.Bacteriol.183(4)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Transferase
ProductType:
RecombinantProtein
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