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商品描述
Species:Mouse
HostSpecies:E.coli
MW:58kDa
Genbank#:NM_011099
Tag(s):N-terminalHis-tag
a.a:2–end
Background:
PKM1andPKM2aredifferentsplicingproductsofthePKMgene(exon9forPKM1andexon10forPKM2).TheM1isoform(PKM1)isexpressedinmuscleandbrain,andtheM2isoformisexpressedduringembryogenesis,inADIposetissueandpancreaticislets,andisthepredominantformfoundincancercells.
Description:
MurinePyruvatekinaseM2(PKM2),alsoknownasPKM1/M2,isoforma(GenBankAccessionNo.NM_011099),a.a.2-end,withN-terminalHis-tag,MW=58kDa,expressedinanE.coliexpressionsystem.
UniProt
P52480
Synonym(s):
pyruvatekinasemuscle,PKM2,cytosolicthyroidhormone-bindingprotein,CTHBP,thyroidhormone-bindingprotein1,THBP1,opa-interactingprotein3,OIP3
SpecificActivity:
≥11pmol/min/µg.
AssayConditions:
A50µlPKM2reactionisconductedinabuffercontaining50mMTris(pH7.4),100mMMgCl2,250mMKCl,40mMADP,100mMphosphoenolpyruvate(PEP)atroomtemperaturefor15min.ATPproductionisdetectedusingKinase-GloLuminescentKinaseAssayPlatform.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and200mMimidazole
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.SpodenG.A.etal.,Exp.CellRes.315(16),2765-2774(2009).
2.ChristofkH.R.etal.,Nature452(7184),230-233(2008).
2.ChristofkH.R.etal.,Nature452(7184),230-233(2008).
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Kinase
ProductType:
RecombinantProtein
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