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商品描述
Species:Human
HostSpecies:E.coli
MW:37.5kDa
Genbank#:NM_002300
Tag(s):N-terminalHis-tag
a.a:2–334(end)
Description:
HumanlactatedehydrogenaseB(LDHB)a.a.2–334(end)(GenBankAccessionNo.NM_002300)withN-terminalHis-tag,MW=37.5kDa,expressedinanE.coliexpressionsystem.
UniProt
P07195
Synonym(s):
lactatedehydrogenaseB,LDHB
SpecificActivity:
≥350nmole/min/µg.
AssayConditions:
Reactionwasconductedin100mMTris,pH7.4,2mMsodiumpyruvate,0.2mMβ-NADH,0.02%BSA,andLDHBinavolumeof200µL.InitiatereactionswithLDHB.MonitorthedecreaseinNADHabsorbanceat340nmat37°Cfor5min.usingTecanInfinite1000.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,240mMimidazole,0.04%Tween-20,and20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Armstrong,A.J.,etal.JClinOncol.2012Sep30;30(27):3402-7.
2.Sudo,K.,etal.,HumGenet.1992May;89(2):158-62.
2.Sudo,K.,etal.,HumGenet.1992May;89(2):158-62.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Dehydrogenase
ProductType:
RecombinantProtein
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