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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:47.6kDa
Genbank#:NM_005896
Tag(s):C-terminalFLAG-tag
a.a:1–414(end)
Description:
HumanIsocitrateDehydrogenase(IDH1),alsoknownasoxalosuccinatedecarboxylase,GenBankAccessionNo.NM_005896,fulllength[a.a.1-414(end)]withC-terminalFLAG-tag,MW=47.6kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
UniProt
O75874
Synonym(s):
IsocitrateDehydrogenase1,IDH1,oxalosuccinatedecarboxylase
SpecificActivity:
≥5600pmol/min/µg.
AssayConditions:
Reactionwasconductedin50mMTris(pH7.4),10mMMgCl2,200µMDL-isocitrate,and200µMNADP.Incubatefor30minutesatroomtemperature.ReadatAbs340usingTecanInfinite1000(5flash/10mssettletime).
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,90µg/mlFLAGpeptide,3mMDTTand10%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Dang,L.,etal.(2010).TrendsMol.Med.16(9):387-97.
2.Paschka,P.,etal.(2010).J.Clin.Oncol.28(22):3636-43.
3.Fu,Y.,etal.(2010).Biochem.Biophys.Res.Commun.397(2):127-30.
2.Paschka,P.,etal.(2010).J.Clin.Oncol.28(22):3636-43.
3.Fu,Y.,etal.(2010).Biochem.Biophys.Res.Commun.397(2):127-30.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Dehydrogenase
ProductType:
RecombinantProtein
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