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商品描述
Species:Human
HostSpecies:E.coli
MW:42kDa
Genbank#:NM_000154
Tag(s):N-terminalHis-tag
a.a:2–399(end)
Description:
Humangalactokinase(GenBankAccessionNo.NM_000154),fulllengthwithN-terminalHis-tag,MW=42kDa,expressedinanE.coliexpressionsystem.
UniProt
P51570
Synonym(s):
GALK1
SpecificActivity:
≥176pmoles/min/µg
AssayConditions:
A50μlGalactokinasereactionisconductedinabuffercontaining20mMTris(pH7.4),5mMMgCl2,50mMNaCl,50μMATP,1mMDTTand1mMsubstrateD-Galactoseatroomtemperaturefor1h.ATPreductionisdetectedusingKinase-Glo®LuminescentKinaseAssayPlatform.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,10%glycerol,225mMimidazole,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.ThodenJ.B.etal.,J.Biol.Chem.280(10),9662-9670(2005).
2.HoldenH.M.etal.,Cell.Mol.LifeSci.61(19-20),2471-2484(2004).
2.HoldenH.M.etal.,Cell.Mol.LifeSci.61(19-20),2471-2484(2004).
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Kinase
ProductType:
RecombinantProtein
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