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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:118.6kDa
Genbank#:NM_145059
Tag(s):N-terminalFLAG-tag
a.a:2–1084(end)
Description:
Humanfucokinase(GenBankAccessionNo.NM_145059),a.a.2-1084(end)withN-terminalFLAG-tag,MW=118.6kDa,expressedinbaculovirususinganSf9insectcellexpressionsystem.
UniProt
Q8N0W3
Synonym(s):
fucokinase,FuK
SpecificActivity:
≥85.3pmole/min/µg
AssayConditions:
Thereactionmixturecontained50mMTris-HCl,pH8.0,5mMMgCl2,2mML-Fucose,100μMATP,andvariousamountoffucokinaseinafinalvolumeof50μL.Thereactionwasincubatedat37°Cfor1hr,andtheamountofATPconsumedwascalculatedusingKinase-GloPlus®reagent(Promega).
Formulation:
40mMTrispH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,3mMDTT,20%glycerol,and80ng/µlFLAGpeptide
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Hinderlich,S.,etal.,BiochemBiophysResCommun.2002Jun14;294(3):650-4.
2.Zeitler,R.,etal.J.Enz.Inhib.1997Feb;11(4):265-73
2.Zeitler,R.,etal.J.Enz.Inhib.1997Feb;11(4):265-73
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Kinase
ProductType:
RecombinantProtein
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